Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens

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• 作者：María J. Salar-García ; Vicente Bernal ; José M. Pastor…
• 关键词：C. salexigens ; Ectoines ; Nitrogen assimilation ; Carbon overflow ; Halophilism ; Fed ; batch
• 刊名：Microbial Cell Factories
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：16
• 期：1
• 全文大小：2002KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
• 出版者：BioMed Central
• ISSN：1475-2859
• 卷排序：16

文摘

BackgroundThe halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of the compatible solutes ectoine and hydroxyectoine. This bacterium has evolved metabolic adaptations to efficiently grow under high salt concentrations by accumulating ectoines as compatible solutes. However, metabolic overflow, which is a major drawback for the efficient conversion of biological feedstocks, occurs as a result of metabolic unbalances during growth and ectoines production. Optimal production of ectoines is conditioned by the interplay of carbon and nitrogen metabolisms. In this work, we set out to determine how nitrogen supply affects the production of ectoines.