Stability of His-Phe-Arg-Trp-Pro-Gly-Pro to Leucine Aminopeptidase, Carboxypeptidase Y, and Rat Nasal Mucus, Blood, and Plasma

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• 作者：K. V. Shevchenko ; S. A. Dulov ; L. A. Andreeva…
• 关键词：semax ; ACTH ; (6–9) ; PGP ; leucine aminopeptidase ; carboxypeptidase Y ; degradation of peptides ; enzymes of nasal mucus ; blood ; and plasma
• 刊名：Russian Journal of Bioorganic Chemistry
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：42
• 期：2
• 页码：153-161
• 全文大小：322 KB
• 参考文献：1.Glazova, N.Yu., Levitskaya, N.G., Andreeva, L.A., Kamenskii, A.A., and Myasoedov, N.F., Dokl. Akad. Nauk, 1999, vol. 367, pp. 137–140.PubMed
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• 作者单位：K. V. Shevchenko (1)
  S. A. Dulov (2)
  L. A. Andreeva (1)
  I. Yu. Nagaev (1)
  V. P. Shevchenko (1)
  A. S. Radilov (2)
  N. F. Myasoedov (1)
  
  1. Institute of Molecular Genetics, Russian Academy of Sciences, plosh. Kurchatova 2, Moscow, 123182, Russia
  2. Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical–Biological Agency, st. Kapitolovo 93, Kuz’molovsky, 188663, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Organic Chemistry
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-330X

文摘

The His-Phe-Arg-Trp-Pro-Gly-Pro [ACTH-(6–9)-PGP] peptide was synthesized. Proteolysis of ACTH-(6–9)-PGP and semax (Met-Glu-His-Phe-Pro-Gly-Pro) in the presence of leucine aminopeptidase and carboxypeptidase Y was studied. If the proteolysis of ACTH-(6–9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. Identification of major metabolites of ACTH-(6–9)PGP when incubated with the enzymes in vitro made it possible to evaluate proteolysis pathways of the peptide and prepare necessary amounts of its metabolites for using them as standards. Kinetics of ACTH-(6–9)PGP degradation in the presence of enzyme systems of nasal mucus, blood, and plasma were also explored. It was found that proteolysis of ACTH-(6–9)-PGP in rat blood and plasma occurs mainly under the effect of enzymes whose action is similar to leucine aminopeptidase.