参考文献:1.Andreeva, A.Yu. and Soldatov, A.A., The sensitivity of nucleated red cells of Scorpena porcus L. to different nitrite concentrations (experiments in vitro), in Mater. III Mezhdunar. ikhtiol. nauch.-prakt. konf. (Proc. III Int. Ichthyol. Sci.-Pract. Conf.), Dnepropetrovsk, 2010, pp. 10鈥?2. 2.Goryachkovskii, A.M., Klinicheskaya biokhimiya v laboratornoi diagnostike (Clinical Biochemistry in the Laboratory Diagnosis), Odessa: Ekologiya, 2005. 3.Maevska, O.M., Boiko, M.M., and Velikii, M.M., Influence of sodium nitrite on catalase activity, Ukr. Biokhim. Zh., 2004, vol. 76, no. 5, pp. 140鈥?43. 4. Metodicheskie ukazaniya po provedeniyu gematologicheskogo obsledovaniya ryb. no. 13-4-2/1487 (Methodological Guidelines for Hematologic Examination of Fish, no. 13-4-2/1487), Moscow: Minsel鈥檏hozprod RF, 1999. 5. Metody otsenki oksidativnogo statusa (Methods of Assessment of Oxidative Status), Voronezh: Izd.-Poligraf. Tsentr Voronezh. Gos. Univ., 2009. 6.Prodanchuk, G.N. and Balan, G.M., Toxic methemoglobinemia: mechanisms of formation and ways to optimize treatment, Sovrem. Problemy Toksikol., 2007, no. 1, pp. 37鈥?5. 7.Soldatov, A.A., Structure, polymorphism, and resistance to oxidation of fish hemoglobins, Zh. Evol. Biokhim. Fiziol., 2002, vol. 38, no. 4, pp. 305鈥?08.PubMed 8.Khuda, L.V., Marchenko, M.M., Khachman, Ya.Yu., and Khudii, O.I., Effect of nitric intoxication on the methemoglobin recovery system in erythrocytes of silver carp, Biol. Sist., 2012, vol. 4, no. 4, pp. 393鈥?96. 9.Khuda, L.V., Marchenko, M.M., and Khudii, O.I., Intensity of oxidative processes in carp erythrocytes under nitric intoxication, Biol. Sist., 2013, vol. 5, no. 1, pp. 16鈥?1. 10.Alexander, J., Benford, D., and Cookburn, A., Nitrite as undesirable substances in animal feed, EFSA J., 2009, vol. 1017, pp. 1鈥?7. 11.Cooper, Ch., Peroxynitrite reacts with methemoglobin to generate globin-bound free radical species, Adv. Exp. Med. Biol., 1999, vol. 454, pp. 195鈥?02. 12.Gy, Papp Zs., Jeney, Zs., and Jeney, G., Comparative studies on the effect of European catfish (Silurus glanis L.) and sturgeon hybrid (Acipenser ruthenus L. 脳 Acipenser baeri L.), J. Appl. Ichthyol., 1995, vol. 11, pp. 372鈥?74.View Article 13.Kroupova, H., Machava, J., and Piackova, V., Nitrite intoxication of common carp (Cyprinus carpio L.) at different water temperatures, Acta Vet. Brno, 2006, vol. 75, pp. 561鈥?69.View Article 14.Moraes, G., Avilez, I.M., and Altran, A.E., Biochemical effects of environmental nitrite in matrinxa (Brycon cephalus), in Aquatic Toxicology: Mechanisms and Consequences. International Congress on the Biology of Fish. Symposium Proceedings, Vancouver, 2002, pp. 15鈥?6. 15.Padayatty, S.J., Katz, A., Wang, Y., et al., Vitamin C as an antioxidant: evaluation of its role in disease prevention, J. Amer. College Nutrit., 2003, vol. 22, no. 1, pp. 18鈥?5.View Article 16.Raja, I.A. and Sapkal, H.P., Blood and electrolyte responses in Clarias batrachus exposed to nitrogen pollution, Biosci. Biotech. Res. Comm., 2011, vol. 4, no. 2, pp. 219鈥?22. 17.Saleh, M.C. and McConkey, S., NADH-dependent cytochrome b5 reductase and NADPH methemoglobin reductase activity in the erythrocytes of Oncorhynchus mykiss, Fish Physiol. Biochem., 2012, vol. 38, no. 6, pp. 1807鈥?813.PubMed View Article 18.Svobodova, Z., Machova, J., and Poleszczuk, G., Nitrite poisoning of fish in aquaculture facilities with water-recirculating system, Acta Vet. Brno, 2000, vol. 74, pp. 129鈥?37.View Article 19.Verlhac, V. and Gabaudan, J., The Effect of Vitamin C on Fish Health. Roche Technical Bulletin, Basel: Hoffmann-La Roche Ltd., 1997.
作者单位:L. V. Khuda (1) O. I. Khudyi (1) M. M. Marchenko (1)
1. Fedkovych National University, Chernivtsi, Ukraine
The content of methemoglobin and the functional status of its recovery system in erythrocytes have been studied for the sterlet Acipenser ruthenus L. exposed to a sodium nitrite concentration ranging from 7.25 to 217.5 mmol/L. The functional features of methemoglobin reductase system form the basis of the specific accumulation of methemoglobin. High concentrations of nitrite in plasma inhibit hemo-containing enzymes (methemoglobin reductase and catalase) that normally prevent the excessive accumulation of methemoglobin. Under the conditions of nitrite intoxication, the leading role in the functioning of the methemoglobin reduction system belongs to its nonenzymatic components (reduced glutathione and ascorbate).