A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1

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• 作者：Ida Barbara Reca (14)
  Vincenzo Lionetti (1)
  Laura Camardella (2)
  Rossana D’Avino (2)
  Thierry Giardina (3)
  Felice Cervone (1)
  Daniela Bellincampi (1) Daniela.bellincampi@uniroma1.it
• 关键词：Pectin methylesterification – Pectin methylesterase inhibitor – Fruit ripening – Solanum lycopersicum
• 刊名：Plant Molecular Biology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：79
• 期：4-5
• 页码：429-442
• 全文大小：845.1 KB
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• 作者单位：1. Dipartimento di Biologia e Biotecnologie “Charles Darwin- “Sapienza-Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy2. Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131 Naples, Italy3. ISM2/BiosCiences UMR CNRS 7313, Faculté des Sciences, Aix-Marseille Université, service 342, 13397 Marseille Cedex 20, France4. Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, USA
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects the activity of plant pectin methylesterases (PMEs) and is inactive against a microbial PME. SolyPMEI transcripts were mainly expressed in flower, pollen and ripe fruit where the protein accumulated at breaker and turning stages of ripening. The expression of SolyPMEI correlated during ripening with that of PME-1, the major fruit specific PME isoform. The interaction of SolyPMEI with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography. The analysis of the zonal distribution of PME activity and the co-localization of SolyPMEI with high esterified pectins suggest that SolyPMEI regulates the spatial patterning of distribution of esterified pectins in fruit.