Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit

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• 作者：Michael A. Cotta (1)
  Terence R. Whitehead (1)
  Enevold Falsen (2)
  Edward Moore (2)
  Paul A. Lawson (3) (4)
  
• 关键词：Enterococci ; 16S rRNA ; Phylogeny ; Taxonomy ; Manure ; Swine
• 刊名：Antonie van Leeuwenhoek
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：103
• 期：1
• 页码：89-98
• 全文大小：442KB
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• 作者单位：Michael A. Cotta (1)
  Terence R. Whitehead (1)
  Enevold Falsen (2)
  Edward Moore (2)
  Paul A. Lawson (3) (4)
  
  1. Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815?N. University Street, Peoria, IL, 61604, USA
  2. Culture Collection, Department of Clinical Bacteriology, University of G?teborg, S-41346, G?teborg, Sweden
  3. Department of Botany and Microbiology, University of Oklahoma, Norman, OK, 73019, USA
  4. Ecology and Evolutionary Biology Program, University of Oklahoma, Norman, OK, 73019, USA
  
• ISSN：1572-9699

文摘

A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacteria isolated from a swine-manure storage pit. On the basis of the 16S rRNA, RNA polymerase α-subunit (rpoA) and 60 kDa chaperonin (cpn60) gene sequence analyses, it was shown that all the isolates were enterococci but formed two separate lines of descent. Pairwise 16S rRNA gene sequence comparisons demonstrated that the two novel organisms were most closely related to each other (97.9?%) and to Enterococcus aquimarinus (97.8?%). Both organisms contained major amounts of C16:0, C16:1 ω7c, C16:1 ω7c, and C18:1 ω7c/12t/9t as the major cellular fatty acids. Based on biochemical, chemotaxonomic and phylogenetic evidence, the names Enterococcus lemanii sp. nov. (type strain PC32T?=?CCUG 61260T?=?NRRL B-59661T) and Enterococcus eurekensis sp. nov. (type strain PC4BT?=?CCUG 61259T?=?NRRL B-59662T) are proposed for these hitherto undescribed species.