Isolation and Characterization of a 尾-Glucosidase from a Clavispora Strain with Potential Applications in Bioethanol Production from Cellulosic Materials
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- 作者：Z. Lewis Liu (1)
Scott A. Weber (1)
Michael A. Cotta (1) - 关键词：Cellobiose ; Cellulosic ethanol ; Simultaneous saccharification and fermentation ; Yeast
- 刊名：Bioenergy Research
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：6
- 期：1
- 页码：65-74
- 参考文献：1. Bothast R, Saha B (1997) Ethanol production from agricultural biomass substrate. Adv Appl Microbiol 44:261鈥?86 CrossRef
2. Wheals AE, Basso LC, Alves DM, Amorim HV (1999) Fuel ethanol after 25聽years. Trends Biotechnol 17:482鈥?87 CrossRef
3. Zaldivar J, Nielsen J, Olsson L (2001) Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. Appl Microbiol Biotechnol 56:17鈥?4 CrossRef
4. Adam AC, Rubio-Texeira M, Polina J (1995) Induced expression of bacterial 尾-glucosidase activity in / Saccharomyces. Yeast 11:395鈥?06 CrossRef
5. Skory CD, Freer SN, Bothast RJ (1996) Expression and secretion of the / Candida wickerhamii extracellular 尾-glucosidase gene bglB in / Saccharomyces cerevisiae. Curr Genet 30:417鈥?22 CrossRef
6. van Rooyen R, Hahn-H盲gerdal B, La Grange DC, van Zyl WH (2005) Construction of cellobiose-growing and fermenting / Saccharomyces cerevisae strains. J Biotechnol 120:284鈥?95 CrossRef
7. Shen Y, Zhang Y, Ma T, Bao X, Du F, Zhuang G et al (2008) Simultaneous saccharification and fermentation of acid-pretreated corncobs with a recombinant / Saccharomyces cerevisiae expressing 尾-glucosidase. Bioresour Technol 99:5099鈥?103 CrossRef
8. Saitoh S, Hasunuma T, Tanaka T, Kondo A (2010) Co-fermentation of cellobiose and xylose using 尾-glucosidase displaying diploid industrial yeast strain OC-2. Appl Microbiol Biotechnol 87:1975鈥?982 CrossRef
9. Gurgu L, Lafraya A, Polaina J, Marin-Navarro J (2011) Fermentation of cellobiose to ethanol by industrial / Saccharomyces strains carrying the 尾-glucosidase gene (BGL1) from / Saccharomycopsis fibuligera. Bioresour Technol 102:5229鈥?236 CrossRef
10. Freer SN (1985) Purification and characterization of the extracellular 尾-glucosidase produced by / Candida wickerhamii. Arch Biochem Biophys 243:515鈥?22 CrossRef
11. Saha BC, Freer SN, Bothast RJ (1994) Production purification and properties of a thermostable 尾-glucosidase from a color variant strain of / Aureobasidium pullulans. Appl Environ Microbiol 60:3774鈥?780
12. Saha BC, Bothast RJ (1996) Production, purification, and characterization of a highly glucose-tolerant novel 尾-glucosidase from / Candida peltata. Appl Environ Microbiol 62:3165鈥?170
13. Barbosa AM, Giese EC, Dekker RFH, Borsato D, Briones P茅rez AI, 脷beda Iranzo JF (2010) Extracellular 尾-glucosidase production by the yeast / Debaryomyces pseudopolymorphus UCLM-NS7A: optimization using response surface methodology. New Biotechnol 27:374鈥?81 CrossRef
14. Liu ZL, Weber SA, Cotta MA, Li S-Z (2012) A new 尾-glucosidase producing yeast for lower-cost cellulosic ethanol production from xylose-extracted corncob residues by simultaneous saccharification and fermentation. Bioresour Technol 104:410鈥?16 CrossRef
15. Grover AK, Macmurche DD, Cushley RJ (1977) Studies on almond emulsion 尾-D-glucosidase I Isolation and characterization of a bifunctional isozyme. Biochem Biophys Acta 482:89鈥?08 CrossRef
16. Saha BC, Biswas A, Cotta MA (2008) Microwave pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. J Biobased Mater Bioenergy 2:210鈥?17 CrossRef
17. Liu ZL, Moon J (2009) A novel NADPH-dependent aldehyde reductase gene from / Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion. Gene 446:1鈥?0 CrossRef
18. Freer SN, Detroy RW (1982) Direct fermentation of cellodextrins to ethanol by / Candida wickerhamii and / C. lusitaniae. Biotechnol Lett 4:453鈥?58 CrossRef
19. Freer SN, Detroy RW (1983) Characterization of cellobiose fermentation to ethanol by yeasts. Biotechnol Bioeng 25:541鈥?57 CrossRef
20. Freer SN, Greene RV (1990) Transport of glucose and cellobiose by / Candida wickerhamii and / Clavispora lusitaniae. J Biol Chem 265:12864鈥?2868
21. Mahajan PM, Desai KM, Lele SS (2010) Production of cell membrane-bound 伪- and 尾-glucosidase from / Lactobacillus acidophilus. Food Bioprocess Technol 5:706鈥?18 CrossRef
22. Skory CD, Freer SN, Bothast RJ (1996) Properties of an intercellular 尾-glucosidase purified from cellobiose-fermenting yeast / Candida wickerhamii. Appl Microbiol Biotechnol 46:353鈥?59
23. Wallecha A, Mishra S (2003) Purification and characterization of two 尾-glucosidases from a thermo-tolerant yeast / Pichia etchellsii. Biochim Biophys Acta 1649:74鈥?4 CrossRef
24. Yapi D, Yapi A, Gnakri D, Niamke SL, Kouame LP (2009) Purification and biochemical characterization of a specific 尾-glucosidase from the digestive fluid of larvae of the palm weevil, / Rhynchophorus palmarum. J Insect Sci 9:4
25. Freer SN (1993) Kinetic Characterization of a 尾-glucosidase from a yeast / Candida wickerhamii. J Biol Chem 268:9337鈥?342
26. Skory CD, Freer SN (1995) Cloning and characterization of a gene encoding a cell-bound extracellular 尾-glucosidase in the yeast / Candida wickerhamii. Appl Environ Microbiol 61:518鈥?25
27. Lee K-M, Jeya M, Joo A-R, Singh R, Kim I-W, Lee J-K (2010) Purification and characterization of a thermostable endo 尾-1, 4-glucanase from novel strain / Penicillium purpurogenum. Enzyme Microb Technol 46:206鈥?11 CrossRef
28. Orkman WE, Day DF (1982) Purification and properties of beta-glucosidase from / Aspergillus terreus. Appl Environ Microbiol 44:1289鈥?295
29. Srivastava SK, Gopalkrishnan KS, Ramachandran KB (1984) Kinetic characterization of a crude beta-d-glucosidase from / Aspergillus wentii Pt 2804. Enzyme Microb Technol 6:508鈥?12 CrossRef
30. Kantham L, Jagannathan V (1985) Beta-Glucosidase of / Penicillium funiculosum. II. Properties and mycelial binding. Biotechnol Bioeng 23:786鈥?91 CrossRef
31. Kitpreechavanich V, Hayashi M, Nagai S (1986) Purification and characterization of extracellular beta-xylosidase and beta-glucosidase from / Aspergillus fumigates. Agric Biol Chem 50:1703鈥?711 CrossRef
32. Chen H, Hayn M, Esterbauer H (1992) Purification and characterization of two extracellular beta-glucosidases from / Trichoderma reesei. Biochim Biophys Acta 1121:54鈥?0 CrossRef
33. Ohnishi M, Okada G, Yazaki T (1998) Characterization of the subsite structure of the beta glucosidase from / Aspergillus niger, an aspect of the mechanism of carbohydrate recognition. Carbohydr Res 308:201鈥?05 CrossRef
34. Hong J, Tamaki H, Kumagai H (2006) Unusual hydrophobic linker region of beta-glucosidase (BGLII) from / Thermoascus aurantiacus is required for hyper-activation by organic solvents. Appl Microbiol Biotechnol 73:80鈥?8 CrossRef
35. Langston J, Sheehy N, Xu F (2006) Substrate specificity of / Aspergillus oryzae family 3 beta glucosidase. Biochim Biophys Acta 1764:972鈥?78 CrossRef
36. Chen M, Qin Y, Liu Z, Liu K, Wang F, Qu Y (2010) Isolation and characterization of a 尾-glucosidase from / Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulose supplementation. Enzyme Microb Technol 46:444鈥?49 CrossRef
37. Decker CH, Visser J, Schreier P (2000) Beta-Glucosidases from five black / Aspergillus species: study of their physico-chemical and biocatalytic properties. J Agric Food Chem 48:4929鈥?936 CrossRef
38. Chauve M, Mathis H, Huc D, Casanave D, Monet F, Ferreira NL (2010) Comparative kinetic analysis of two fungal 尾-glucosidases. Biotechnol Biofuels 3:3 CrossRef
39. Kristensen JB, Felby C, J酶rgensen H (2009) Yield-determining factors in high-solids enzymatic hydrolysis of lignocelluloses. Biotechnol Biofuels 2:11 CrossRef - 作者单位：Z. Lewis Liu (1)
Scott A. Weber (1)
Michael A. Cotta (1)
1. Bioenergy Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA - ISSN：1939-1242
文摘
We previously reported on a new yeast strain of Clavispora sp. NRRL Y-50464 that is capable of utilizing cellobiose as sole source of carbon and energy by producing sufficient native 尾-glucosidase enzyme activity without further enzyme supplementation for cellulosic ethanol production using simultaneous saccharification and fermentation. Eliminating the addition of external 尾-glucosidase reduces the cost of cellulosic ethanol production. In this study, we present results on the isolation and identification of a 尾-glucosidase protein from strain Y-50464. Using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and blast search of the NCBInr database (National Center for Biotechnology Information nonredundant), the protein from Y-50464 was identified as a 尾-glucosidase (BGL1) with a molecular weight of 93.3聽kDa. The BGL1 protein was purified through multiple chromatographic steps to a 26-fold purity (K m鈥?鈥?.355聽mM [pNPG]; K i鈥?鈥?5.2聽mM [glucose]), which has a specific activity of 18.4聽U/mg of protein with an optimal performance temperature at 45聽掳C and pH of 6.0. This protein appears to be intracellular although other forms of the enzyme may exist. The fast growth rate of Y-50464 and its capability to produce sufficient 尾-glucosidase activity for ethanol conversion from cellobiose provide a promising means for low-cost cellulosic ethanol production through a consolidated bioprocessing development.