Simultaneous chromatography and electrophoresis: two-dimensional planar separations

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• 作者：Peter R. Stevenson (1)
  Bret E. Dunlap (1)
  Paul S. Powell (1)
  Brae V. Petersen (1)
  Christopher J. Hatch (1)
  Hung Chan (1)
  Garret I. Still (1)
  Michael T. Fulton (1)
  Justin S. McKell (1)
  David C. Collins (1)
  
• 关键词：Planar chromatography ; Amino acids ; Separations/instrumentation ; Electrophoresis
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：405
• 期：10
• 页码：3085-3089
• 全文大小：309KB
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• 作者单位：Peter R. Stevenson (1)
  Bret E. Dunlap (1)
  Paul S. Powell (1)
  Brae V. Petersen (1)
  Christopher J. Hatch (1)
  Hung Chan (1)
  Garret I. Still (1)
  Michael T. Fulton (1)
  Justin S. McKell (1)
  David C. Collins (1)
  
  1. Department of Chemistry, Brigham Young University鈥擨daho, 525 South Center Street, Rexburg, ID, 83460, USA
  
• ISSN：1618-2650

文摘

Single-dimension separations are routinely coupled in series to achieve two-dimensional separations, yet little has been done to simultaneously exploit multiple dimensions during separation. In this work, simultaneous chromatography and electrophoresis is introduced and evaluated for its potential to achieve two-dimensional separations. In simultaneous chromatography and electrophoresis, chromatography occurs via capillary action while an orthogonal electric field concurrently promotes electrophoresis in a second dimension. A novel apparatus with a dual solvent reservoir was designed to apply the concurrent electric field. Various compounds were used to characterize the apparatus and technique, i.e., vitamins, amino acids, and dyes. Improved separation is reported with equivalent analysis times in comparison to planar chromatography alone. The feasibility of simultaneously employing chromatography and electrophoresis in two dimensions is discussed. Figure Separation of eight dyes is improved in comparison to (a) planar chromatography alone when employing (b) simultaneous chromatography and electrophoresis