Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice

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• 作者：Asheesh Gupta (1) (2) (3) r> Tianhong Dai (1) (2) r> Michael R. Hamblin (1) (2) (4) r>
• 关键词：Cytokeratin ; 14 ; Low ; level laser (light) therapy ; Near ; infrared ; Partial ; thickness abrasion ; Photobiomodulation ; Proliferating cell nuclear antigen (PCNA)
• 刊名：Lasers in Medical Science
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：29
• 期：1
• 页码：257-265
• 全文大小：517 KB
• 作者单位：Asheesh Gupta (1) (2) (3) r> Tianhong Dai (1) (2) r> Michael R. Hamblin (1) (2) (4) r>r>1. BAR414, Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA, 02114, USA r> 2. Department of Dermatology, Harvard Medical School, Boston, MA, 02115, USA r> 3. Defence Institute of Physiology & Allied Sciences, Delhi, 110 054, India r> 4. Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA r>
• ISSN：1435-604X

文摘

Low-level laser (light) therapy (LLLT) promotes wound healing, reduces pain and inflammation, and prevents tissue death. Studies have explored the effects of various radiant exposures on the effect of LLLT; however, studies of wavelength dependency in in vivo models are less common. In the present study, the healing effects of LLLT mediated by different wavelengths of light in the red and near-infrared (NIR) wavelength regions (635, 730, 810, and 980?nm) delivered at constant fluence (4?J/cm2) and fluence rate (10?mW/cm2) were evaluated in a mouse model of partial-thickness dermal abrasion. Wavelengths of 635 and 810?nm were found to be effective in promoting the healing of dermal abrasions. However, treatment using 730- and 980-nm wavelengths showed no sign of stimulated healing. Healing was maximally augmented in mice treated with an 810-nm wavelength, as evidenced by significant wound area reduction (p-lt;-.05), enhanced collagen accumulation, and complete re-epithelialization as compared to other wavelengths and non-illuminated controls. Significant acceleration of re-epithelialization and cellular proliferation revealed by immunofluorescence staining for cytokeratin-14 and proliferating cell nuclear antigen (p-lt;-.05) was evident in the 810-nm wavelength compared with other groups. Photobiomodulation mediated by red (635?nm) and NIR (810?nm) light suggests that the biological response of the wound tissue depends on the wavelength employed. The effectiveness of 810-nm wavelength agrees with previous publications and, together with the partial effectiveness of 635?nm and the ineffectiveness of 730 and 980?nm wavelengths, can be explained by the absorption spectrum of cytochrome c oxidase, the candidate mitochondrial chromophore in LLLT.