Microflora of phytopathogen-transferring Bradysia agrestis: a step toward finding ideal candidates for paratransgenesis
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- 作者：Young-Hyun You ; Jong Myong Park ; Pyoung-Ho Yi ; Chang-Gi Back ; Mi-Jeoung Park…
- 关键词：Vector borne disease ; Paratransgenesis ; Bradysia agrestis ; Bacillus aryabhattai ; Symbiotic microorganisms
- 刊名：Symbiosis
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：71
- 期：1
- 页码：35-46
- 全文大小：
- 刊物主题：Evolutionary Biology; Developmental Biology; Microbiology; Plant Sciences; Ecology;
- 出版者：Springer Netherlands
- ISSN：1878-7665
- 卷排序：71
文摘
Owing to the effect of symbiotic gut bacteria on the proliferation of infectious disease-transferring insects and their potential as paratransgenic tools, their identification or securing their use in controlling insect vectors is urgently needed. Bradysia agrestis is one of the major plant epidemic vectors in East Asia; however, its normal microbiota remains unstudied. This research was primarily designed to secure promising candidates for use in a paratransgenic approach to phytopathogen control, as well as to provide a picture of the gut microbiota of wild-caught B. agrestis from a blight field. A total of 180 larvae from three different regions in South Korea were analyzed for their gut microfloral composition. Diverse bacterial strains were purely isolated based on morphological differences in microbiological media. We found a total of 64 bacterial strains in the B. agrestis midgut and analyzed their 16S rRNA gene sequences for molecular identification and phylogenetic characterization. Furthermore, the microbial diversity of each normal microbiota group was analyzed with various diversity indices. Bacterial biota including species diversity, richness or species distribution, species constituents, and dominance varied by each regional vector group. The most widely distributed species was Bacillus aryabhattai, and the most common genera were Bacillus and Microbacterium. These saprophytic bacteria were isolated from all regional groups and their potential as paratransgenic candidates for controlling B. agrestis-mediated plant disease is discussed.