Improvement of immunoassay detection sensitivity by using well-defined raspberry-like magnetic microbeads as carriers
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- 作者：Ye-fei Wang (1)
Xin Zhang (1)
Ping Xu (1)
Hong-chen Gu (1)
Hong Xu (1) - 关键词：immunoassay ; raspberry ; like ; magnetic microbeads ; detection sensitivity ; R 318.08 ; TB 333
- 刊名：Journal of Shanghai Jiaotong University (Science)
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：19
- 期：5
- 页码：538-543
- 全文大小：697 KB
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Xin Zhang (1)
Ping Xu (1)
Hong-chen Gu (1)
Hong Xu (1)
1. School of Biomedical Engineering; Med-X Research Institute, Shanghai Jiaotong University, Shanghai, 200030, China - ISSN：1995-8188
文摘
Well-defined raspberry-like magnetic microbeads (RMMBs) as immunoassay solid carriers were prepared by chemical covalent binding between Fe3O4 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles (3O4@SiO2 core-shell magnetic microbeads (CMMBs) with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank (LoB) and limit of detection (LoD) of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay (CLIA) were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.