Analysis of cytomixis in tobacco microsporocytes with confocal laser scanning microscopy

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• 作者：Sergey Mursalimov ; Yuri Sidorchuk ; Elena Deineko
• 关键词：Cytomixis ; Confocal laser scanning microscopy ; Nicotiana tabacum ; Cell wall
• 刊名：Protoplasma
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：254
• 期：1
• 页码：539-545
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Cell Biology; Plant Sciences; Zoology;
• 出版者：Springer Vienna
• ISSN：1615-6102
• 卷排序：254

文摘

Confocal laser scanning microscopy for the first time is used to examine the structure of the tobacco microsporocytes involved in the intercellular migration of nuclei (cytomixis). As is observed, the cytomictic channels are distributed over the surface of tobacco microsporocytes in a non-random manner and their number depends on the meiotic stage. Analysis of non-squash cells demonstrates the differences in cytological patterns of cytomixis in a normal meiosis of control tobacco plants (SR1 line) and the abnormal meiosis of polyploids. As a rule, two to three adjacent cells are involved in cytomixis during meiosis of control tobacco plants; after cytomixis, several micronuclei are formed in recipient cells; cytoplasts (enucleated cells) are rare; and polyads are undetectable. In the meiosis of polyploids, cytomixis is massive, with a larger number of cells (sometimes, over ten) involved in nuclear migration simultaneously; recipient cells on completion of cytomixis develop tens of micronuclei; cytoplasts and polyads are frequently detectable.