Evaluating genetic biodiversity of Parkia biglobosa (Jacq.) R. Br. Ex Don (African locust bean) accessions from Nigeria using seed protein electrophoresis

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• 作者：Adenubi I. Adesoye ; Kayode A. Apo
• 关键词：African locust bean ; genetic diversity ; Nigeria ; PAGE ; Parkia biglobosa ; Seed protein fractions
• 刊名：Journal of Crop Science and Biotechnology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：18
• 期：3
• 页码：171-180
• 全文大小：1,208 KB
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• 作者单位：Adenubi I. Adesoye (1)
  Kayode A. Apo (1)
  
  1. Department of Botany, University of Ibadan, Ibadan, Nigeria
  
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：2005-8276

文摘

Thirty-four accessions of African locust bean (Parkia biglobosa) collected from different localities across Nigeria were screened for variability in seed storage proteins. Globulin, albumin, and vicilin fractions were investigated by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and native PAGE. Both methods of analysis revealed a substantial degree of heterogeneity in the protein fractions. However, native PAGE produced fewer protein bands and profiles than SDS-PAGE. Although accessions exhibited significant similarity in their polypeptide profiles, differences in migration rate and staining intensities as well as presence / absence of specific bands accounted for variations obtained in the accessions examined. The absence of 10 kDa albumin subunit was specific to accessions 24, 25, 26, and 29 while the presence of 30.4 kDa vicilin was unique to accessions 5, 6, and 7. One major cluster of globulin band between two polypeptides 42.7 -50 kDa, and two minor bands 31.4 and 16.2 kDa were observed. Protein fraction profiles were in most cases not similar among the accessions collected from same agroecological zone. This suggests that gene flow between the different zones must have occurred due to movement of germplasm and exchange of genetic material among farmers and markets. Groupings based on cluster analysis and polypeptide patterns agree and confirm the presence of duplicates within the accessions studied. Keywords African locust bean genetic diversity Nigeria PAGE Parkia biglobosa Seed protein fractions