Recombinant expression, purification, and characterization of an acyl-CoA binding protein from Aspergillus oryzae

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• 作者：Qing Hao ; Xiaoguang Liu ; Guozhong Zhao ; Lu Jiang ; Ming Li…
• 关键词：Acyl ; CoA binding affinity ; Acyl ; CoA binding protein ; Aspergillus oryzae ; Microscale thermophoresis binding assay ; Myristoyl ; CoA ; Palmitoyl ; CoA
• 刊名：Biotechnology Letters
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：38
• 期：3
• 页码：519-525
• 全文大小：1,252 KB
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• 作者单位：Qing Hao (1)
  Xiaoguang Liu (2)
  Guozhong Zhao (3)
  Lu Jiang (4)
  Ming Li (1)
  Bin Zeng (4)
  
  1. Key Laboratory of Industrial Fermentation Microbiology, National Engineering Laboratory for Industrial Enzymes, College of Biotechnology, Ministry of Education, Tianjin University of Science & Technology, Tianjin, 300457, China
  2. College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology, Tianjin, 300457, China
  3. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China
  4. College of Life Science, Jiangxi Science & Technology Normal University, Nanchang, 330013, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objectives To characterize biochemically the lipid metabolism-regulating acyl-CoA binding protein (ACBP) from the industrially-important fungus Aspergillus oryzae.