Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding
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- 作者：Jong-il Choi (1)
Minchul Yoon (2)
Minho Joe (2)
Hyun Park (3) (4)
Sung Gu Lee (3) (4)
Se Jong Han (3) (4)
Pyung Cheon Lee (5) - 关键词：Scenedesmus dimorphus ; Radiation breeding ; Lipid content ; 2 ; Dimensional electrophoresis
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：37
- 期：12
- 页码：2437-2444
- 全文大小：1,139 KB
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Minchul Yoon (2)
Minho Joe (2)
Hyun Park (3) (4)
Sung Gu Lee (3) (4)
Se Jong Han (3) (4)
Pyung Cheon Lee (5)
1. Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 500-757, South Korea
2. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, South Korea
3. Division of Life Sciences, Korea Polar Research Institute, Incheon, 406-840, South Korea
4. Department of Polar Sciences, Korea University of Science and Technology, Incheon, 406-840, South Korea
5. Department of Molecular Science and Technology and Department of Applied Chemistry and Biological Engineering, Ajou University, Suwon, South Korea - ISSN：1615-7605
文摘
In this study, a high lipid-accumulating mutant strain of the microalgae Scenedesmus dimorphus was developed via radiation breeding. To induce mutant strain, S. dimorphus was gamma-irradiated at doses from 100 to 800?Gy, and then a mutant (Sd-Pm210) with 25?% increased lipid content was selected using Nile red staining methodology. Sd-Pm210 showed morphological changes and had higher growth rate compared to the wild type. From random amplified polymorphic DNA analysis, partial genetic modifications were also observed in Sd-Pm210. In comparisons of lipid content between wild type and Sd-Pm210 using thin-layer chromatography, the content of triacylglycerol was markedly higher in the Sd-Pm210 strain. The total peak area of fatty acid methyl ester was shown to have about 1.4-fold increase in Sd-Pm210, and major fatty acids were identified as palmitic acid, oleic acid, linoleic acid, and linolenic acid. To define the metabolic changes in the mutant strain, 2-dimensional electrophoresis was conducted. Several proteins related to lipid synthesis and energy metabolisms were overexpressed in the mutant strain. These results showed that radiation breeding can be utilized for the development of efficient microalgae strains for biofuel production.