Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

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• 作者：Zhaowei Cai ; Haitao Xi ; Yongming Pan ; Xiaoling Jiang…
• 关键词：Testosterone ; Atherosclerosis ; Hypercholesterolemia ; Miniature pigs ; Gene expression
• 刊名：Lipids in Health and Disease
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：14
• 期：1
• 全文大小：1,046 KB
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• 刊物主题：Lipidology; Medical Biochemistry;
• 出版者：BioMed Central
• ISSN：1476-511X

文摘

Background Testosterone deficiency is associated with increased serum cholesterol levels. However, how testosterone deficiency precisely affects cholesterol metabolism remains unclear. Therefore, in the current study, we examined the effect of testosterone deficiency on cholesterol metabolism and liver gene expression in pigs fed a high-fat and high-cholesterol (HFC) diet. Methods Sexually mature male miniature pigs (6- months old) were randomly divided into 3 groups as follows: intact male pigs fed an HFC diet (IM-?HFC), castrated male pigs fed an HFC diet (CM-?HFC), and castrated pigs with testosterone replacement fed an HFC diet (CM-?HFC-?T). Serum testosterone levels and lipid profiles were measured, and gene expression levels associated with hepatic cholesterol metabolism were determined. Furthermore, total hepatic cholesterol contents and the activities of enzymes mediating hepatic cholesterol metabolism were measured. Results Serum testosterone levels were significantly decreased in CM-?HFC pigs, and testosterone replacement attenuated castration-induced testosterone deficiency. Castration significantly increased the serum levels of total cholesterol, low-density lipoprotein cholesterol and triglycerides, as well as hepatic lipid contents in pigs fed an HFC diet. Compared with IM-?HFC and CM-?HFC-?T pigs, low-density lipoprotein receptor (LDLR) mRNA expression and protein levels were significantly decreased in the livers of CM-?HFC pigs. In contrast, we found that compared with IM-?HFC pigs, hepatic proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA and serum PCSK9 protein levels were significantly increased in CM-?HFC pigs. Moreover, testosterone treatment reversed the increase in PCSK9 expression in CM-?HFC pigs. However, neither castration nor testosterone replacement affected the expression of the other hepatic genes that were tested. Conclusions This study demonstrated that castration-induced testosterone deficiency caused severe hypercholesterolemia in pigs fed an HFC diet; furthermore, these effects could be reversed by testosterone replacement therapy. Altered hepatic PCSK9 and LDLR expression, resulting in reduced LDL-cholesterol clearance, may contribute to the increased serum cholesterol levels induced by testosterone deficiency and an HFC diet. These results deepen our understanding of the underlying molecular mechanisms that mediate the effects of testosterone deficiency on cholesterol metabolism.