Molecular characterization of Beclin 1 in rare minnow (Gobiocypris rarus) and its expression after waterborne cadmium exposure
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- 作者：Xiao-Hong Liu ; Zhi-Jian Wang ; Dong-Ming Chen…
- 关键词：Beclin 1 ; cDNA cloning ; Rare minnow (Gobiocypris rarus) ; Cadmium exposure
- 刊名：Fish Physiology and Biochemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：42
- 期：1
- 页码：111-123
- 全文大小：2,594 KB
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Zhi-Jian Wang (1)
Dong-Ming Chen (1)
Mu-Fei Chen (1)
Xing-Xing Jin (1)
Jing Huang (1)
Yao-Guang Zhang (1)
1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, 400715, China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Hydrobiology
Animal Physiology
Animal Anatomy, Morphology and Histology
Animal Biochemistry
Zoology - 出版者：Springer Netherlands
- ISSN：1573-5168
文摘
Beclin 1 plays an important role in autophagy and apoptosis which are well documented in mammals. However, relevant reports are rare in fish. This study characterized Beclin 1 of the rare minnow Gobiocypris rarus (rmBeclin 1), which encodes a peptide of 447 amino acids using RT-PCR and RACE. The deduced peptide showed 96.4 and 80.8 % similarity to Beclin 1 of common carp and human, respectively. Semiquantitative RT-PCR revealed that rmBeclin 1 was ubiquitously expressed in all tested tissues of male and female fish in all developmental stages, even unfertilized eggs. RT-qPCR revealed that rmBeclin 1 mRNA transcripts were significantly up-regulated in gills after a 12 h treatment with waterborne CdCl2 but were decreased thereafter. However, rmBeclin 1 expression was decreased in the brain, but it was not significantly changed in other tissues. Subchronic CdCl2 exposure significantly increased rmBeclin 1 in the brain, but it distinctly decreased rmBeclin 1 in the gill and hepatopancreas. A dose-dependent effect was not observed in mature fish treated for 96 h, but a dose-dependent effect existed in immature fish treated for 10 days. Longer treatment (10 day) caused a significantly higher expression of rmBeclin 1 in the larvae groups. These data suggest that alterations in rmBeclin 1 after CdCl2 exposure are tissue-specific and time-related and that the dose-dependent effect was restricted to a certain concentration range and exposure time. Keywords Beclin 1 cDNA cloning Rare minnow (Gobiocypris rarus) Cadmium exposure