Berberine attenuates oxidative stress and hepatocytes apoptosis via protecting mitochondria in blunt snout bream Megalobrama amblycephala fed high-fat diets
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- 作者：Kang-Le Lu ; Li-Na Wang ; Ding-Dong Zhang ; Wen-Bin Liu…
- 关键词：High ; fat diet ; Oxidative stress ; Apoptosis ; Mitochondrial function ; Berberine ; Blunt snout bream
- 刊名：Fish Physiology and Biochemistry
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：43
- 期：1
- 页码：65-76
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Freshwater & Marine Ecology; Animal Physiology; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Zoology;
- 出版者：Springer Netherlands
- ISSN：1573-5168
- 卷排序：43
文摘
High-fat diets may have favorable effects on growth and cost, but high-fat diets often induce excessive fat deposition, resulting in liver damage. This study aimed to identify the hepatoprotective of a Chinese herb (berberine) for blunt snout bream (Megalobrama amblycephala). Fish were fed with a normal diet (LFD, 5 % fat), high-fat diet (HFD, 15 % fat) or berberine-supplemented diets (BSD, 15 % fat with berberine 50 or 100 mg/kg level) for 8 weeks. After the feeding, histology, oxidative status and mitochondrial function of liver were assessed. The results showed that HFD caused fat accumulation, oxidative stress and apoptosis in hepatocytes of fish. Hepatocytes in HFD group appeared to be hypertrophied, with larger liver cells diameter than these of LFD group. Berberine-supplemented diets could attenuate oxidative stress and hepatocytes apoptosis. HFD induced the decreasing mitochondrial complexes activities and bulk density and surface area density. Berberine improved function of mitochondrial respiratory chain via increasing the complex activities. Moreover, the histological results showed that berberine has the potential to repair mitochondrial ultrastructural damage and elevate the density in cells. In conclusion, our study demonstrated that berberine has attenuated liver damage induced by the high fat mainly via the protection for mitochondria.