Effects of manganese deficiency on chondrocyte development in tibia growth plate of Arbor Acres chicks

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• 作者：Jian Wang ; Zhen Yong Wang ; Zhao Jun Wang ; Ran Liu…
• 关键词：Manganese deficiency ; Chondrocytes ; Proliferation ; Apoptosis
• 刊名：Journal of Bone and Mineral Metabolism
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：33
• 期：1
• 页码：23-29
• 全文大小：1,870 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Metabolic Diseases
  Orthopedics
  Internal Medicine
  
• 出版者：Springer Japan
• ISSN：1435-5604

文摘

The aim of this study was to investigate the effects of manganese (Mn) deficiency on chondrocyte development in tibia growth plate. Ninety 1-day-old Arbor Acres chicks were randomly divided into three groups and fed on control diet (60?mg Mn/kg diet) and manganese deficient diets (40?mg Mn/kg diet, manganese deficiency group I; 8.7?mg Mn/kg diet, manganese deficiency group II), respectively. The width of the proliferative zone of growth plate was measured by the microscope graticule. Chondrocyte apoptosis was estimated by TUNEL staining. Gene expression of p21 and Bcl-2, and expression of related proteins were analyzed by quantitative real time reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Compared with the control group, manganese deficiency significantly decreased the proliferative zone width and Bcl-2 mRNA expression level, while significantly increased the apoptotic rates and the expression level of p21 gene in chondrocytes. The results indicate that manganese deficiency had a negative effect on chondrocyte development, which was mediated by the inhibition of chondrocyte proliferation and promotion of chondrocyte apoptosis.