Gallic Acid-g-Chitosan Modulates Inflammatory Responses in LPS-Stimulated RAW264.7 Cells Via NF-κB, AP-1, and MAPK Pathways
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- 作者：Chang-Bum Ahn ; Won-Kyo Jung ; Sun-Joo Park ; Yong-Tae Kim ; Won-Suk Kim…
- 关键词：chitosan ; RAW macrophage ; inflammation ; NF ; κB ; AP ; 1 ; MAPK
- 刊名：Inflammation
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：39
- 期：1
- 页码：366-374
- 全文大小：1,479 KB
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Won-Kyo Jung (2)
Sun-Joo Park (3)
Yong-Tae Kim (4)
Won-Suk Kim (5)
Jae-Young Je (6)
1. Division of Food and Nutrition, Chonnam National University, Gwangju, 550-757, Republic of Korea
2. Department of Biomedical Engineering, Pukyong National University, Busan, 608-737, Republic of Korea
3. Department of Chemistry, Pukyong National University, Busan, 608-737, Republic of Korea
4. Department of Food Science and Biotechnology, Kunsan National University, Kunsan, 573-701, Republic of Korea
5. Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University, Busan, 46958, Republic of Korea
6. Department of Marine-Bio Convergence Science, Pukyong National University, Busan, 608-739, Republic of Korea - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Rheumatology
Internal Medicine
Pharmacology and Toxicology
Pathology - 出版者：Springer Netherlands
- ISSN：1573-2576
文摘
Chitosan is a naturally occurring polysaccharide, which has exhibited antioxidant, antimicrobial, and anti-cancer activities among others. Modification of chitosan by grafting phenolic compounds is a good strategy for improvement of bioactivities of chitosan. We investigated the anti-inflammatory action of gallic acid-grafted-chitosan (GAC) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. GAC inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) by inhibiting inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages. GAC also suppressed the production and mRNA expression of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). GAC inactivated nuclear factor-κB (NF-κB) via inhibiting the phosphorylation and degradation of the NF-κB inhibitor, IκB. In addition, GAC suppresses the activation of activator protein-1 (AP-1) through the phosphorylation of mitogen-activated protein kinase (MAPK) such as extracellular signal-regulated kinase (ERK1/2), p38 MAPK, and c-Jun N-terminal kinase/stress-activated protein kinase (JNK). These results suggest that GAC has the potential anti-inflammatory action by downregulating transcriptional factors (NF-κB and AP-1) through MAPK signaling pathways. KEY WORDS chitosan RAW macrophage inflammation NF-κB AP-1 MAPK