Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain

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• 作者：Thomas Amort ; Dietmar Rieder ; Alexandra Wille ; Daria Khokhlova-Cubberley…
• 关键词：RNA methylation ; 5 ; Methylcytosine ; m5C ; Epitranscriptome ; Embryonic stem cells ; Mouse brain ; m6A ; RNA binding proteins ; Bisulfite sequencing ; meRIP
• 刊名：Genome Biology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：18
• 期：1
• 全文大小：2100KB
• 刊物主题：Animal Genetics and Genomics; Human Genetics; Plant Genetics & Genomics; Microbial Genetics and Genomics; Bioinformatics; Evolutionary Biology;
• 出版者：BioMed Central
• ISSN：1474-760X
• 卷排序：18

文摘

BackgroundRecent work has identified and mapped a range of posttranscriptional modifications in mRNA, including methylation of the N6 and N1 positions in adenine, pseudouridylation, and methylation of carbon 5 in cytosine (m5C). However, knowledge about the prevalence and transcriptome-wide distribution of m5C is still extremely limited; thus, studies in different cell types, tissues, and organisms are needed to gain insight into possible functions of this modification and implications for other regulatory processes.