Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry
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- 作者：Donato Koyalta (28)
Mohammad-Ali Jenabian (29)
Barou Djouater (30)
No?l Djemadji-Oudjeil (31)
Francois-Xavier Mbopi-Keou (32)
Angélique Ndjoyi-Mbiguino (33)
Laurent Bélec (34) - 关键词：Flow cytometry ; CD4 T cell count ; CD45 ; Decentralization ; Sub ; Saharan Africa ; Chad
- 刊名：BMC Health Services Research
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：13
- 期：1
- 全文大小：372 KB
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37. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6963/13/373/prepub - 作者单位：Donato Koyalta (28)
Mohammad-Ali Jenabian (29)
Barou Djouater (30)
No?l Djemadji-Oudjeil (31)
Francois-Xavier Mbopi-Keou (32)
Angélique Ndjoyi-Mbiguino (33)
Laurent Bélec (34)
28. Ministère de la Santé Publique, BP 407, N’Djamena, Chad
29. Chronic Viral Illness Service and Research Institute, McGill University Health Centre, Montreal, Canada
30. Conseil National de Lutte contre le SIDA, Primature, N’Djamena, Chad
31. Organisation Mondiale de la Santé, Représentation du Tchad, N’Djamena, Chad
32. National Public Health Laboratory, Ministry of Public Health, University of Yaounde I, Yaounde, Cameroon
33. Laboratoire National de Référence des Maladies Sexuellement Transmissibles et du SIDA, Département de Microbiologie, Faculté de Médecine de Libreville, Université des Sciences de la Santé, Libreville, Gabon
34. Laboratoire de virologie, h?pital Européen Georges Pompidou, and Faculté de Médecine Paris Descartes, Université Paris Descartes (Paris V), Sorbonne Paris Cité, Paris, France - ISSN：1472-6963
文摘
Background Field and community evaluation of the routine usage of CD4 T counting platforms is essential in resource-poor countries for efficient and cost-effective monitoring of HIV-infected adults and children attending health care centers. Methods We herein addressed the principal issues raised by the implementation of the single-platform, volumetric Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) in 8 community HIV monitoring laboratories of different levels throughout Chad. This is a country with particularly difficult conditions, both in terms of climate and vast geographical territory, making the decentralization of the therapeutic management of HIV-infected patients challenging. Results The routine usage of the Auto40 flow cytometers for a period of 5?years (2008-013) confirms the reliability and robustness of the analyzer for community-based CD4 T cell enumeration in terms of both absolute numbers and percentages to enable accurate monitoring of HIV-infected adults and children. However, our observations suggest that the Auto40 mini flow cytometer is not suitable for all laboratories as it is oversized and ultimately very expensive. Conclusion The Chad experience with the Auto40 flow cytometer suggests that its usage in resource-limited settings should be mainly reserved to reference (level 1) or district (level 2) laboratories, rather than to laboratories of health care centres (level 3).