Application of the consolidated species concept to identify the causal agent of strawberry anthracnose in Iran and initial molecular dating of the Colletotrichum acutatum species complex

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• 作者：Kaivan Karimi ; Asadollah Babai Ahari ; Mahdi Arzanlou…
• 关键词：Colletotrichum nymphaeae ; Introduction ; Molecular clock ; Multi ; gene approach ; Kurdistan
• 刊名：European Journal of Plant Pathology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：147
• 期：2
• 页码：375-387
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Pathology; Plant Sciences; Ecology; Agriculture; Life Sciences, general;
• 出版者：Springer Netherlands
• ISSN：1573-8469
• 卷排序：147

文摘

Anthracnose caused by Colletotrichum spp. is one of main strawberry diseases worldwide. The disease recently arrived in Iran and become epidemic in the Kurdistan province. A combination of morphological, molecular and pathogen-host interaction approaches (referred to the consolidated species concept) were applied to isolates from symptomatic strawberry tissues collected in Iran. Multi-gene phylogenetic analysis based on ITS-rDNA, beta-tubulin (TUB2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) identified the species as the S5 clade of Colletotrichum nymphaeae. No significant intraspecies variation was observed in terms of morphology and pathogenicity. No significant genetic variation was observed among the isolates using inter simple sequence repeat analysis (ISSR) and primer pair combination of ERIC1/BOX and ERIC2/BOX fingerprinting markers. Based on the results of this study, it can be postulated that the C. nymphaeae population in Iran established itself from a single origin due to a founder effect. More generally, molecular dating based on relaxed clocks indicates co-radiation of C. nymphaeae S5 and strawberry plants and suggests high reciprocal specificity between the host and pathogen.