Isolation a P450 gene in Pinus armandi and its expression after inoculation of Leptographium qinlingensis and treatment with methyl jasmonate

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• 作者：T. Pham ; H. Chen ; L. Dai ; T. Q. T. Vu
• 关键词：Pinus armandi ; Leptographium qinlingensis ; cytochrome P450 monooxygenase ; methyl jasmonate
• 刊名：Russian Journal of Plant Physiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：63
• 期：1
• 页码：111-118
• 全文大小：1,176 KB
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• 作者单位：T. Pham (1) (2)
  H. Chen (1)
  L. Dai (1)
  T. Q. T. Vu (1)
  
  1. College of Forestry, Northwest A&F University, Yangling, Shaanxi, P.R. China
  2. Hue University’s College of Education, Hue, Vietnam
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3407

文摘

In conifers, the cytochrome P450 monooxygenase of the CYP720B family plays an important role in the synthesis of diterpene resin acids, which are specialised metabolites of the oleoresin defence. We isolated CYP720B19 from Pinus armandi and found that it belongs to the CYP720B family. The CYP720B19 open reading frame of 1467 bp encodes a protein of 488 amino acid residues with high similarity to abietadienol/abietadienal oxidase. Differential expression of the CYP720B19 gene significantly changed when plants were inoculated with the fungus Leptographium qinlingensis, mechanically wounded, or treated with methyl jasmonate (MeJa) and Tween at 4 days and 8 days. Increased expression of the CYP720B19 gene after these treatments suggested that the gene was involved in pine defence against inoculation by L. qinlingensis, MeJa treatment and mechanical injury.