文摘
The phototherapy effects in the skin are related to biomodulation, usually to accelerate wound healing. However, there is no direct proof of the interrelation between the effects of low-level laser therapy (LLLT) and light-emitting diode (LED) in neuropeptide secretion, these substances being prematurely involved in the neurogenic inflammation phase of wound healing. This study therefore focused on investigating LLLT and LED in Calcitonin gene-related peptide (CGRP) and substance P (SP) secretion in healthy rat skin. Forty rats were randomly distributed into five groups with eight rats each: Control Group, Blue LED Group (470?nm, 350?mW power), Red LED Group (660?nm, 350?mW power), Red Laser Group (660?nm, 100?mW power), and Infrared Laser Group (808?nm, 100?mW power) (DMC? Equipamentos Ltda., S?o Carlos, S?o Paulo, Brazil). The skin of the animals in the experimental groups was irradiated using the punctual contact technique, with a total energy of 40?J, single dose, standardized at one point in the dorsal region. After 14?min of irradiation, the skin samples were collected for CGRP and SP quantification using western blot analysis. SP was released in Infrared Laser Group (p--.01); there was no difference in the CGRP secretion among groups. Infrared (808?nm) LLLT enhances neuropeptide SP secretion in healthy rat skin.