Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort ( Leonurus japonicus )
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- 作者：Xingyong Yang ; Xiaowen Wang ; Xianbi Li ; Beibei Zhang ; Yuehua Xiao ; Demou Li ; Chengjian Xie and Yan Pei
- 关键词：Expression ; Disease resistance ; Antimicrobial protein ; Phylopenetic analysis ; Transgenic tobacco
- 刊名：Plant Cell Reports
- 出版年：2008
- 出版时间：April, 2008
- 年：2008
- 卷：27
- 期：4
- 页码：759-766
- 全文大小：445.1 KB
文摘
In screening for potent antimicrobial proteins (AMPs) from plant seeds, we had purified a heat-stable AMP, LJAMP2, from the seeds of a medicine herb, motherwort (Leonurus japonicus Houtt). In an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. Then a cDNA encoding LJAMP2 was cloned by the rapid amplification of cDNA ends based on the N-terminal amino acid sequence determined. The deduced amino acid sequences of this cDNA show similarity to plant non-specific lipid transfer proteins. Northern blotting assay revealed that this nsLTP-like gene, designated LJAMP2, was expressed in seeds. Overexpression of LJAMP2 in tobacco enhanced resistance to the fungal pathogen Alternaria alternata and the bacterial pathogen Ralstonia solanacearum, significantly, while no visible alteration in plant growth and development. Our data confirm the antifungal and antibacterial function of LJAMP2 from motherwort seeds and suggest the potential of LJAMP2 in improving disease resistance in plants.