Assessing the effect of a nude mouse model on nanoparticle-mediated gene delivery
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- 作者：Jamie L. Betker ; Thomas J. Anchordoquy
- 关键词：Immunocompetent ; Nude mice ; Immunocompromised ; Gene delivery ; Lipoplex ; Biodistribution
- 刊名：Drug Delivery and Translational Research
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：7
- 期：1
- 页码：162-167
- 全文大小：
- 刊物主题：Pharmaceutical Sciences/Technology;
- 出版者：Springer US
- ISSN：2190-3948
- 卷排序：7
文摘
The relevance of using nude mouse models for evaluating drug delivery to human tumors has recently been questioned by numerous researchers. While the immune response is known to play a critical role in cancer, this study assesses the effect of using immunocompromised “nude” mice on drug delivery. By inoculating both nude and immunocompetent mice with a mouse mammary carcinoma cell line (4T1), differences in the “first pass effect”, distribution, and reporter gene expression due to the use of the nude mouse model could be elucidated. Our results indicate that initial tumor deposition (5 min) was slightly lower in nude mice but comparable after 24 h. In addition, some small differences in tissue deposition/accumulation and reporter gene expression were observed between the two mouse models. The results with this one tumor model suggest that delivery studies conducted in nude mice can provide comparable results to those in immunocompetent mouse models.