Improved multiplex PCR assay for simultaneous detection of Bacillus cereus emetic and enterotoxic strains
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- 作者：Jae-Myung Kim (1)
Fereidoun Forghani (1)
Jung-Beom Kim (2)
Yong-Bae Park (2)
Myoung-Su Park (1)
Jun Wang (1)
Joong Hyun Park (1)
Deog-Hwan Oh (1) - 关键词：multiplex PCR ; Bacillus cereus ; emetic toxin ; enterotoxin ; food poisoning
- 刊名：Food Science and Biotechnology
- 出版年：2012
- 出版时间：October 2012
- 年：2012
- 卷：21
- 期：5
- 页码：1439-1444
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Fereidoun Forghani (1)
Jung-Beom Kim (2)
Yong-Bae Park (2)
Myoung-Su Park (1)
Jun Wang (1)
Joong Hyun Park (1)
Deog-Hwan Oh (1)
1. Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea
2. Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment, Suwon, Gyeonggi, 440-290, Korea - ISSN：2092-6456
文摘
Toxin producing Bacillus cereus can cause enterotoxic and/or emetic food poisoning. In the present study, a multiplex PCR assay was developed to detect all toxin genes known to be involved in food poisoning of B. cereus in a single reaction. Specific primers for the detection of enterotoxic (entFM, hblC, nheA, and cytK) genes and emetic toxin production (2 primer pairs: ces, CER) were designed based on the GeneBank sequences. The developed multiplex PCR assay was evaluated in pure culture and artificially inoculated milk, using 43 B. cereus strains and non-target strains. In brief, sensitivity in pure culture was 10-fold or more higher than artificially inoculated milk in multiplex PCR detection limit assay. The presented PCR assay is a developed molecular tool for the rapid simultaneous detection of emetic and enterotoxin producing B. cereus strains.