Size distribution of allergenic Cry j 2 released from airborne Cryptomeria japonica pollen grains during the pollen scattering seasons

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• 作者：Xiumin Gong ; Qingyue Wang ; Senlin Lu ; Miho Suzuki ; Daisuke Nakajima&#8230
• 关键词：Japanese cedar pollinosis ; Cryptomeria japonica pollen ; Size ; segregated allergenic species ; Surface plasmon resonance (SPR)
• 刊名：Aerobiologia
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：33
• 期：1
• 页码：59-69
• 全文大小：
• 刊物类别：Earth and Environmental Science
• 刊物主题：Atmospheric Protection/Air Quality Control/Air Pollution; Environmental Health; Plant Pathology; Allergology; Pneumology/Respiratory System; Environmental Engineering/Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1573-3025
• 卷排序：33

文摘

Japanese cedar (Cryptomeria japonica) pollinosis is one of seasonal allergic rhinitis that mainly occurs in Japan. The pollinosis is caused by two main kinds of allergenic proteins called Cry j 1 and Cry j 2 which exist in Cryptomeria japonica pollen. In our previous study, we reported that the size-segregated of airborne fine allergenic Cry j 1 and morphological change of Cry j 1 due to the contact with rainfall. However, the study on airborne allergenic Cry j 2 in different particle sizes has not been identified until now. Therefore, the main aim of this study is to investigate the size distribution and scattering behavior of allergenic Cry j 2. The Cry j 2 particles were collected and determined in different particle sizes at the urban sampling points during the most severe pollination season of 2012 in Saitama, Japan. After the size-segregated Cry j 2 allergenic particles were collected using an Andersen high-volume (AHV) atmospheric sample, the airborne Cry j 2 concentrations were determined with a surface plasmon resonance (SPR) method. At the same time, the airborne Cryptomeria japonica pollens were also counted by the Durham pollen sampler. The higher concentrations of the allergenic Cry j 2 were detected even in particle sizes equal to or less than 1.1 μm (PM_1.1) than other particle sizes. The airborne particles ranges from 0.06 to 11 μm were also collected by a low-pressure impactor (LPI) atmospheric sampler. After that, the concentrations of Cry j 2 allergenic particles in fine particle sizes were measured by the SPR method either. With the help of this study, we have confirmed the existence of fine daughter allergenic particles, which clearly differ from the parent pollen grains in size, especially after the rainy days. It is possible that the daughter allergenic species will be released from the fractions of cell wall and burst pollen grains. We concluded that rainwater was one of the important factors that affects the release of pollen allergenic proteins of both Cry j 1 and Cry j 2 from the parent pollen grains.