Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells

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• 作者：K. Srikanth ; Tito Trindade ; A. C. Duarte…
• 关键词：Chinook salmon ; Iron oxide nanoparticles ; Cytotoxicity ; Oxidative stress ; Lipid peroxidation ; Protein carbonyl
• 刊名：Environmental Science and Pollution Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：24
• 期：2
• 页码：2055-2064
• 全文大小：
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water M
• 出版者：Springer Berlin Heidelberg
• ISSN：1614-7499
• 卷排序：24

文摘

The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe₃O₄ NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe₃O₄ NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 μg/ml concentration of Fe₃O₄ NPs after 24 h. Fe₃O₄ NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 μg/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe₃O₄ NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.