Biofabrication of Cubic Phase Silver Nanoparticles Loaded with Phytochemicals from Solanum nigrum Leaf Extracts for Potential Antibacterial, Antibiofilm and Antioxidant Activities Against MDR Human Pathogens

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• 作者：U. Jinu ; N. Jayalakshmi ; A. Sujima Anbu ; D. Mahendran…
• 关键词：Antibacterial effect ; Antibiofilm activity ; Antioxidant efficacy ; Multidrug resistant pathogens ; Solanum nigrum ; Silver nanoparticles
• 刊名：Journal of Cluster Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：489-505
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Catalysis; Inorganic Chemistry; Physical Chemistry; Nanochemistry;
• 出版者：Springer US
• ISSN：1572-8862
• 卷排序：28

文摘

The present report describes an efficient method for bioengineering and characterization of silver nanoparticles (AgNPs) using aqueous leaf extracts of Solanum nigrum and its antibacterial as well as antibiofilm activity against multidrug resistant (MDR) human pathogens. The leaf extract was prepared using microwave irradiation method and used as potential reducing as well as stabilizing agent for synthesis of AgNPs. The formation of silver nanoparticles was monitored by UV–Visible spectra analysis at 428 nm. Fourier transform infrared spectroscopy analysis confirmed the presence of various functional biomolecules loaded on AgNPs and XRD results indicated the crystalline nature of the synthesized silver nanoparticles. Bioengineered silver nanoparticles were found to be cuboidal shape detected by scanning electron microscopy analysis. EDX spectrum showed the presence of strong signal for silver ions in the nanoparticles. The average particle size was found to be 20 nm. The synthesized silver nanoparticles exhibited potential antibacterial as well as antioxidant activity against Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonous aeruginosa, Klebsiella pneumoniae and Proteus valgaris. In addition, biofabricated AgNPs showed significantly higher rate of antibiofilm activity against two different biofouling bacterial strains viz., P. aeruginosa and S. epidermidis. The present study highlighted the effective method for the synthesis of biomolecules-loaded silver nanoparticles and their potential antibacterial, antioxidant as well as antibiofilm activity against gram-positive and gram-negative clinically isolated human pathogens. Results showed that bioengineered AgNPs could be used as nanodrug for the treatment of MDR bacterial infections caused by biofilm with high efficiency.