An integrative Raman microscopy-based workflow for rapid in situ analysis of microalgal lipid bodies
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- 作者：Sudhir Kumar Sharma ; David R. Nelson ; Rasha Abdrabu…
- 关键词：Algae ; Biofuel ; Single cell analysis ; FACS ; Confocal Raman microscopy ; Lipidomics
- 刊名：Biotechnology for Biofuels
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：8
- 期：1
- 全文大小：2,269 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
- 出版者：BioMed Central
- ISSN：1754-6834
文摘
Background Oils and bioproducts extracted from cultivated algae can be used as sustainable feedstock for fuels, nutritional supplements, and other bio-based products. Discovery and isolation of new algal species and their subsequent optimization are needed to achieve economical feasibility for industrial applications. Here we describe and validate a workflow for in situ analysis of algal lipids through confocal Raman microscopy. We demonstrate its effectiveness to characterize lipid content of algal strains isolated from the environment as well as algal cells screened for increased lipid accumulation through UV mutagenesis combined with Fluorescence Activated Cell Sorting (FACS).