Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum

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• 作者：Chenghua Li (1)
  Lingling Wang (2)
  Xuanxuan Ning (3)
  Aiqin Chen (1)
  Linbao Zhang (1)
  Song Qin (1)
  Huifeng Wu (1)
  Jianmin Zhao (1)
  
• 关键词：Venerupis philippinarum ; Small heat shock protein ; Time鈥揷ourse expression ; Vibrio anguillarum ; Cadmium exposure
• 刊名：Cell Stress and Chaperones
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：15
• 期：6
• 页码：897-904
• 全文大小：1041KB
• 参考文献：1. Gao Q, Song L, Ni D, Wu L, Zhang H, Chang Y (2007) cDNA cloning and mRNA expression of heat shock protein 90 gene in the haemocytes of Zhikong scallop / Chlamys farreri. Comp Biochem Physiol 147B:704鈥?15
  2. Haslbeck M (2002) sHsps and their role in the chaperone network. Cell Mol Life Sci 59:1649鈥?657 CrossRef
  3. Hickman-Miller HD, Hildebrand WH (2004) The immune response under stress: the role of HSP-derived peptides. Trends Immunol 25:427鈥?33 CrossRef
  4. Huang P, Kang S, Chen W, Hsu T, Lo C, Liu K, Chen L (2008) Identification of the small heat shock protein, HSP21, of shrimp / Penaeus monodon and the gene expression of HSP21 is inactivated after white spot syndrome virus (WSSV) infection. Fish Shellfish Immunol 25:250鈥?57 CrossRef
  5. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150鈥?63 CrossRef
  6. Li Z, Menoret A, Srivastava P (2002) Roles of heat-shock proteins in antigen presentation and cross-presentation. Curr Opin Immunol 14:45鈥?1 CrossRef
  7. Li C, Sun H, Chen A, Ning X, Wu H, Qin S, Xue Q, Zhao J (2009) Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam / Venerupis philippinarum. Fish Shellfish Immunol. doi:10.1016/j.fsi.2009.11.021
  8. Lindquist S, Craig EA (1988) The heat-shock proteins. Annu Rev Genet 22:631鈥?77 CrossRef
  9. Mehlen P, Schulze-Osthoff K, Arrigo AP (1996) Small stress proteins as novel regulators of apoptosis. J Biol Chem 271:16510鈥?6514 CrossRef
  10. Mizrahi T, Heller J, Goldenberg S, Arad Z (2009) Heat shock proteins and resistance to desiccation in congeneric land snails. Cell Stress Chaperon. doi:10.1007/s12192-009-0150-9
  11. Morrow G, Inaguma Y, Kato K, Anguay RM T (2000) The small heat shock protein hsp22 of / Drosophila melanogaster is a mitochondrial protein displaying oligomeric organization. J Biol Chem 275:31204鈥?1210 CrossRef
  12. Moseley P (2000) Stress proteins and the immune response. Immunopharmacology 48:299鈥?02 CrossRef
  13. Oshita SE, Chen F, Kwan T, Yehiely F, Cryns VL (2010) The small heat shock protein HspB2 is a novel anti-apoptotic protein that inhibits apical caspase activation in the extrinsic apoptotic pathway. Breast Cancer Res Treat. doi:10.1007/s10549-010-0735-0
  14. Pockley AG (2003) Heat shock proteins as regulators of the immune response. Lancet 362:469鈥?76 CrossRef
  15. Pomerai D (1996) Heat-shock proteins as biomarkers of pollution. Hum Exp Toxicol 15:279鈥?85 CrossRef
  16. Sol茅 M, Morcillo Y, Porte C (2000) Stress-protein response in tributyltin-exposed clams. Bull Environ Contam Toxicol 64:852鈥?58 CrossRef
  17. S酶rensen JG, Kristensen TN, Loeschcke V (2003) The evolutionary and ecological role of heat shock proteins. Ecol Lett 6:1025鈥?037 CrossRef
  18. Song L, Wu L, Ni D, Chang Y, Xu W, Xing K (2006) The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop ( / Argopecten irradians, Lamarck 1819) responding to bacteria challenge and naphthalin stress. Fish Shellfish Immunol 21:335鈥?45 CrossRef
  19. Srivastava PK (2002) Role of heat-shock proteins in innate and adaptive immunity. Nat Rev Immunol 2:185鈥?94 CrossRef
  20. Thomas X, Campos L, Le Q, Guyotat D (2005) Heat shock proteins and acute leukemias. Hematology 10:225鈥?35 CrossRef
  21. Tsan MF, Gao B (2004a) Heat shock protein and innate immunity. Cell Mol Immunol 1:274鈥?79
  22. Tsan MF, Gao B (2004b) Cytokine function of heat shock proteins. Am J Physiol 286:C739鈥揅744 CrossRef
  23. Tsvetkova NM, Horv谩th I, Zs T, Wolkers WF, Balogi Z, Shigapova N, Crowe LM, Tablin F, Vierling E, Crowe JH, V铆gh L (2002) Small heat-shock proteins regulate membrane lipid polymorphism. Proc Natl Acad Sci USA 99:13504鈥?3509 CrossRef
  24. Wallin RP, Lundqvist A, More SH, Bonin A, Kiessling R, Ljunggren HG (2002) Heat shock proteins as activators of the innate immune system. Trends Immunol 23:l30鈥?35 CrossRef
  25. Woolfson JP, Heikkila JJ (2009) Examination of cadmium-induced expression of the small heat shock protein gene, / hsp30, in / Xenopus laevis A6 kidney epithelial cells. Comp Biochem Physiol 152A:91鈥?9
  26. Zhang L, Wang L, Song L, Zhao J, Qiu L, Dong C, Li F, Zhang H, Yang G (2009a) The involvement of HSP22 from bay scallop / Argopecten irradians in response to heavy metal stress. Mol Biol Rep. doi:10.1007/s11033-009-9603-6
  27. Zhang L, Wang L, Zhao J, Qiu L, Song L, Dong C, Li F (2009b) The responsive expression of heat shock protein 22 gene in Zhikong scallop / Chlamys farreri against a bacterial challenge. Aquac Res 41:257鈥?66 CrossRef
  
• 作者单位：Chenghua Li (1)
  Lingling Wang (2)
  Xuanxuan Ning (3)
  Aiqin Chen (1)
  Linbao Zhang (1)
  Song Qin (1)
  Huifeng Wu (1)
  Jianmin Zhao (1)
  
  1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Laishan District, 264003, Yantai, People鈥檚 Republic of China
  2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People鈥檚 Republic of China
  3. Yantai Oceanic Environmental Monitoring Central Station of SOA, Yantai, 264006, People鈥檚 Republic of China
  
• ISSN：1466-1268

文摘

Small heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm.