RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid

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• 作者：Jian-Xin Liu ; Chung-Yi Chiou ; Chin-Hui Shen ; Peng-Jen Chen ; Yao-Chung Liu…
• 关键词：Carotenoid ; Oncidium orchid ; Phytoene synthase ; Plastid ; RNA interference
• 刊名：SpringerPlus
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：3
• 期：1
• 全文大小：1992KB
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• 作者单位：Jian-Xin Liu (6)
  Chung-Yi Chiou (10) (7)
  Chin-Hui Shen (7) (9)
  Peng-Jen Chen (7)
  Yao-Chung Liu (7)
  Chin-Der Jian (8)
  Xiao-Lan Shen (6)
  Fu-Quan Shen (6)
  Kai-Wun Yeh (7)
  
  6. Flower Research and Development Center, Zhejiang Academy of Agricultural Science, Hangzhou, 311202, Zhejiang, China
  10. Institute of Bioinformatics and Structural Biology & Department of Life Science, National Tsing Hua University, 101, Sec. 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan
  7. Institute of Plant Biology, College of Life Science, National Taiwan University, Roosevelt Road, Taipei, 10617, Taiwan
  9. Ecological Materials Technology Department, Green Energy & Eco-technology System Center, ITRI South Campus, Industrial Technology Research Institute, Tainan, Taiwan
  8. Institute of Forestry Research, Council of Agriculture, Taipei, Taiwan
  
• 刊物类别：Science, general;
• 刊物主题：Science, general;
• 出版者：Springer International Publishing
• ISSN：2193-1801

文摘

Phytoene synthase (PSY) is the first rate-limiting regulatory enzyme in the carotenoid biosynthesis pathway. In order to modify the floral color pattern by reducing carotenoid contents, a phytoene synthase-RNAi construct was delivered into protocorm-like body (PLB) of Oncidium hybrid orchid. The transgenic orchids show down-regulated level of PSY and geranyl synthase gene. They displayed semi-dwarf phenotype and brilliant green leaves. The microscopic anatomy revealed development-arrested plastids with rare grana. The total carotenoid content was decreased and the efficiency of the photosynthetic electron transport was declined. The chlorophyll level and the expression of chlorophyll biosynthetic genes, such as OgGLUTR and OgCS were dramatically reduced. HPLC analysis showed that the endogenous level of gibberellic acid and abscisic acid in the dwarf transformants are 4-fold lower than in wild type plants. In addition, chilling tolerance of the transgenic Oncidium plants was reduced. The data showed that down-regulation of PSY resulted in alterations of gene expression in enzymes involved in many metabolic pathways, such as carotenoid, gibberellic acid, abscisic acid and chlorophyll biosynthetic pathway as well as causes predominant defects in plant growth and development. Keywords Carotenoid Oncidium orchid Phytoene synthase Plastid RNA interference