Impact of tightly focused femtosecond laser pulses on nucleolus-like bodies of mouse GV oocyte and the ability of mouse oocytes to mature

详细信息    查看全文

• 作者：A. A. Astafev ; A. D. Zalesskiy ; O. V. Zatsepina…
• 刊名：Doklady Biochemistry and Biophysics
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：467
• 期：1
• 页码：136-140
• 全文大小：980 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Biophysics and Biomedical Physics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3091
• 卷排序：467

文摘

Using femtosecond laser radiation, nucleolus-like bodies (NLBs) of mouse oocytes were locally dissected without damage to zona pellucida, cytoplasmic membrane, nuclear membrane, and nucleoplasm surrounding NLB. It was found that, after dissection of 2.7 × 10–11 cm3 of NLB material, which is approximately 5.2% of 10 μm NLB volume, the probability of germinal vesicle oocyte development to metaphase II stage of meiosis decreased 3–7 times compared to the non-treated oocytes. This result indicates that NLB material organization is significant for mouse oocyte maturation.Original Russian Text © A.A. Astafev, A.D. Zalesskiy, O.V. Zatsepina, A.N. Kostrov, A.S. Krivoharchenko, A.A. Osychenko, G.A. Serobyan, V.A. Nadtochenko, 2016, published in Doklady Akademii Nauk, 2016, Vol. 467, No. 5, pp. 602–606.Presented by Academician V.A. Shuvalov October 21, 2015