Exposure to the complement C5b-9 complex sensitizes 661W photoreceptor cells to both apoptosis and necroptosis

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• 作者：Hui Shi (1) (3)
  Jennifer A. E. Williams (1)
  Li Guo (2)
  Dimitrios Stampoulis (1)
  M. Francesca Cordeiro (2)
  Stephen E. Moss (1)
  
  1. Department of Cell Biology ; UCL Institute of Ophthalmology ; 11-43 Bath Street ; London ; EC1V 9EL ; UK
  3. Department of Ophthalmology ; Tongji Hospital ; Tongji Medical School ; Huazhong University of Science and Technology ; Wuhan ; China
  2. Department of Visual Neuroscience ; UCL Institute of Ophthalmology ; 11-43 Bath Street ; London ; EC1V 9EL ; UK
  
• 关键词：661W ; Apoptosis ; Necroptosis ; Complement ; c5b ; 9 ; Retina
• 刊名：Apoptosis
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：20
• 期：4
• 页码：433-443
• 全文大小：2,087 KB
• 参考文献：1. Noris, M, Remuzzi, G (2013) Overview of complement activation and regulation. Semin Nephrol 33: pp. 479-492 CrossRef
  2. Ballanti, E, Perricone, C, Greco, E (2013) Complement and autoimmunity. Immunol Res 56: pp. 477-491 422-y" target="_blank" title="It opens in new window">CrossRef
  3. Stevens, B, Allen, NJ, Vazquez, LE (2007) The classical complement cascade mediates CNS synapse elimination. Cell 131: pp. 1164-1178 CrossRef
  4. Stephan, AH, Barres, BA, Stevens, B (2012) The complement system: an unexpected role in synaptic pruning during development and disease. Annu Rev Neurosci 35: pp. 369-389 46/annurev-neuro-061010-113810" target="_blank" title="It opens in new window">CrossRef
  5. Kim, DD, Song, WC (2006) Membrane complement regulatory proteins. Clin Immunol 118: pp. 127-136 4" target="_blank" title="It opens in new window">CrossRef
  6. Fett, AL, Hermann, MM, Muether, PS (2012) Immunohistochemical localization of complement regulatory proteins in the human retina. Histol Histopathol 27: pp. 357-364
  7. Williams, JA, Greenwood, J, Moss, SE (2013) Retinal changes precede visual dysfunction in the complement factor H knockout mouse. PLoS ONE 8: pp. e68616 CrossRef
  8. Faber, C, Williams, J, Juel, HB (2012) Complement factor H deficiency results in decreased neuroretinal expression of Cd59a in aged mice. Invest Ophthalmol Vis Sci 53: pp. 6324-6330 CrossRef
  9. Sancho-Pelluz, J, Arango-Gonzalez, B, Kustermann, S (2008) Photoreceptor cell death mechanisms in inherited retinal degeneration. Mol Neurobiol 38: pp. 253-269 45-9" target="_blank" title="It opens in new window">CrossRef
  10. Murakami, Y, Miller, JW, Vavvas, DG (2011) RIP kinase-mediated necrosis as an alternative mechanism of photoreceptor death. Oncotarget 2: pp. 497-509
  11. Marigo, V (2007) Programmed cell death in retinal degeneration: targeting apoptosis in photoreceptors as potential therapy for retinal degeneration. Cell Cycle 6: pp. 652-655 4161/cc.6.6.4029" target="_blank" title="It opens in new window">CrossRef
  12. Kanan, Y, Moiseyev, G, Agarwal, N (2007) Light induces programmed cell death by activating multiple independent proteases in a cone photoreceptor cell line. Invest Ophthalmol Vis Sci 48: pp. 40-51 CrossRef
  13. Bowes Rickman C, Farsiu S, Toth CA et al (2013) Dry age-related macular degeneration: mechanisms, therapeutic targets, and imaging. Invest Ophthalmol Vis Sci 54:ORSF68-80
  14. Krishnamoorthy, RR, Clark, AF, Daudt, D (2013) A forensic path to RGC-5 cell line identification: lessons learned. Invest Ophthalmol Vis Sci 54: pp. 5712-5719 CrossRef
  15. Moskovich, O, Herzog, LO, Ehrlich, M (2012) Caveolin-1 and dynamin-2 are essential for removal of the complement C5b-9 complex via endocytosis. J Biol Chem 287: pp. 19904-19915 4/jbc.M111.333039" target="_blank" title="It opens in new window">CrossRef
  16. Cashman, SM, Desai, A, Ramo, K (2011) Expression of complement component 3 (C3) from an adenovirus leads to pathology in the murine retina. Invest Ophthalmol Vis Sci 52: pp. 3436-3445 CrossRef
  17. Bovellan, M, Fritzsche, M, Stevens, C (2010) Death-associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death. FEBS J 277: pp. 58-65 42-4658.2009.07412.x" target="_blank" title="It opens in new window">CrossRef
  18. Hamann, S, M茅trailler, S, Schorderet, DF (2013) Analysis of the cytoprotective role of 伪-crystallins in cell survival and implication of the 伪A-crystallin C-terminal extension domain in preventing Bax-induced apoptosis. PLoS ONE 8: pp. e55372 CrossRef
  19. Daudt, DR, Yorio, T (2011) FKBP51 protects 661w cell culture from staurosporine-induced apoptosis. Mol Vis 17: pp. 1172-1181
  20. Gani, OA, Engh, RA (2010) Protein kinase inhibition of clinically important staurosporine analogues. Nat Prod Rep 27: pp. 489-498 48b" target="_blank" title="It opens in new window">CrossRef
  21. Outeiro, TF, Kontopoulos, E, Altmann, SM (2007) Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson鈥檚 disease. Science 317: pp. 516-519 43780" target="_blank" title="It opens in new window">CrossRef
  22. Narayan, N, Lee, IH, Borenstein, R (2012) The NAD-dependent deacetylase SIRT2 is required for programmed necrosis. Nature 492: pp. 199-204 CrossRef
  23. Chinskey, ND, Besirli, CG, Zacks, DN (2014) Retinal cell death and current strategies in retinal neuroprotection. Curr Opin Ophthalmol 25: pp. 228-233 43" target="_blank" title="It opens in new window">CrossRef
  24. Lueck, K, Wasmuth, S, Williams, J (2011) Sub-lytic C5b-9 induces functional changes in retinal pigment epithelial cells consistent with age-related macular degeneration. Eye (Lond) 25: pp. 1074-1082 CrossRef
  25. Yang, P, Baciu, P, Kerrigan, BC (2014) Retinal pigment epithelial cell death by the alternative complement cascade: role of membrane regulatory proteins, calcium, PKC, and oxidative stress. Invest Ophthalmol Vis Sci 55: pp. 3012-3021 4" target="_blank" title="It opens in new window">CrossRef
  26. Morgan, BP (1989) Complement membrane attack on nucleated cells: resistance, recovery and non-lethal effects. Biochem J 264: pp. 1-14
  27. Chang, Q, Berdyshev, E, Cao, D (2014) Cytochrome P450 2C epoxygenases mediate photochemical stress-induced death of photoreceptors. J Biol Chem 289: pp. 8337-8352 4/jbc.M113.507152" target="_blank" title="It opens in new window">CrossRef
  28. Zhou, WT, Ni, YQ, Jin, ZB (2012) Electrical stimulation ameliorates light-induced photoreceptor degeneration in vitro via suppressing the proinflammatory effect of microglia and enhancing the neurotrophic potential of M眉ller cells. Exp Neurol 238: pp. 192-208 CrossRef
  29. Han, J, Zhong, CQ, Zhang, DW (2011) Programmed necrosis: backup to and competitor with apoptosis in the immune system. Nat Immunol 12: pp. 1143-1149 CrossRef
  30. Vandenabeele, P, Galluzzi, L, Vanden Berghe, T (2010) Molecular mechanisms of necroptosis: an ordered cellular explosion. Nat Rev Mol Cell Biol 11: pp. 700-714 CrossRef
  31. Simenc, J, Lipnik-Stangelj, M (2012) Staurosporine induces different cell death forms in cultured rat astrocytes. Radiol Oncol 46: pp. 312-320 478/v10019-012-0036-9" target="_blank" title="It opens in new window">CrossRef
  32. Cordeiro, MF, Guo, L, Coxon, KM (2010) Imaging multiple phases of neurodegeneration: a novel approach to assessing cell death in vivo. Cell Death Dis 1: pp. e3 CrossRef
  33. Shulga, N, Pastorino, JG (2012) GRIM-19-mediated translocation of STAT3 to mitochondria is necessary for TNF-induced necroptosis. J Cell Sci 125: pp. 2995-3003 42/jcs.103093" target="_blank" title="It opens in new window">CrossRef
  34. Moujalled, DM, Cook, WD, Okamoto, T (2013) TNF can activate RIPK3 and cause programmed necrosis in the absence of RIPK1. Cell Death Dis 4: pp. e465 CrossRef
  35. Genini, S, Beltran, WA, Aguirre, GD (2013) Up-regulation of tumor necrosis factor superfamily genes in early phases of photoreceptor degeneration. PLoS ONE 8: pp. e85408 408" target="_blank" title="It opens in new window">CrossRef
  36. Sato, K, Li, S, Gordon, WC, He, J (2013) Receptor interacting protein kinase-mediated necrosis contributes to cone and rod photoreceptor degeneration in the retina lacking interphotoreceptor retinoid-binding protein. J Neurosci 33: pp. 17458-17468 CrossRef
  37. Faber C, Jehs T, Juel HB et al (2014) Early and exudative age-related macular degeneration is associated with increased plasma levels of soluble TNF receptor II. Acta Ophthalmol. doi: 10.1111/aos.12581 . [Epub ahead of print]
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

The loss of photoreceptors is the defining characteristic of many retinal degenerative diseases, but the mechanisms that regulate photoreceptor cell death are not fully understood. Here we have used the 661W cone photoreceptor cell line to ask whether exposure to the terminal complement complex C5b-9 induces cell death and/or modulates the sensitivity of these cells to other cellular stressors. 661W cone photoreceptors were exposed to complete normal human serum following antibody blockade of CD59. Apoptosis induction was assessed morphologically, by flow cytometry, and on western blotting by probing for cleaved PARP and activated caspase-3. Necroptosis was assessed by flow cytometry and Sirtuin 2 inhibition using 2-cyano-3-[5-(2,5-dichlorophenyl)-2-furyl]-N-5-quinolinylacrylamide (AGK2). The sensitivity of 661W cells to ionomycin, staurosporine, peroxide and chelerythrine was also investigated, with or without prior formation of C5b-9. 661W cells underwent apoptotic cell death following exposure to C5b-9, as judged by poly(ADP-ribose) polymerase 1 cleavage and activation of caspase-3. We also observed apoptotic cell death in response to staurosporine, but 661W cells were resistant to both ionomycin and peroxide. Interestingly, C5b-9 significantly increased 661W sensitivity to staurosporine-induced apoptosis and necroptosis. These studies show that low levels of C5b-9 on 661W cells can induce apoptosis, and that C5b-9 specifically sensitizes 661W cells to certain apoptotic and necroptotic pathways. Our observations provide new insight into the potential role of the complement system in photoreceptor loss, with implications for the molecular aetiology of retinal disease.