Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice

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• 作者：Masayoshi Yukita ; Shigeki Machida ; Koji M. Nishiguchi…
• 关键词：Axonal injury ; Glaucoma ; Retinal ganglion cell ; Electroretinogram ; Scotopic threshold response
• 刊名：Documenta Ophthalmologica
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：130
• 期：2
• 页码：149-156
• 全文大小：1,988 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Ophthalmology
  
• 出版者：Springer Netherlands
• ISSN：1573-2622

文摘

Purpose Optic nerve crush (ONC) and subsequent axonal damage can be used in rodents to study the mechanism of retinal ganglion cell (RGC) degeneration. Here, we examined electroretinograms (ERGs) in post-ONC mice to investigate changes in the positive scotopic threshold response (pSTR). We then compared these changes with molecular and morphological changes to identify early objective biomarkers of RGC dysfunction. Methods Fifty 12-week-old C57BL/6 mice were included. ONC was used to induce axonal injury in the right eye of each animal, with the left eye used as a control. The expression of the RGC markers Brn3a and Brn3b was measured on days 1, 2, 3, 5 and 7 after ONC with quantitative real-time PCR. ERGs were recorded under dark adaptation with the stimulus intensity increasing from ?.2 to 0.43 log cd-s/m2 on days 1, 2, 3, 5, 7 and 10 after ONC. The pSTR, a- and b-wave amplitudes were measured. Inner retinal thickness around the optic nerve head was measured with spectral-domain optical coherence tomography on days 0, 2, 5, 7 and 10 after ONC. Results The expression of Brn3a and Brn3b began to significantly decrease on day 1 and day 2, respectively (P?P?P? Conclusions Decrease in pSTR likely?reflected the early loss of RGC function after ONC and that declining expression of RGC-specific genes preceded anatomical and functional changes in the RGCs.