Whole genome sequence analysis of multidrug-resistant Mycobacterium tuberculosis Beijing isolates from an outbreak in Thailand

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• 作者：Sanjib Mani Regmi ; Angkana Chaiprasert…
• 关键词：Mycobacterium tuberculosis ; Multidrug resistance ; Outbreak ; Whole genome sequencing ; Thailand
• 刊名：Molecular Genetics and Genomics
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：290
• 期：5
• 页码：1933-1941
• 全文大小：530 KB
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• 作者单位：Sanjib Mani Regmi (1)
  Angkana Chaiprasert (1)
  Supasak Kulawonganunchai (2)
  Sissades Tongsima (2)
  Olabisi Oluwabukola Coker (1)
  Therdsak Prammananan (2)
  Wasna Viratyosin (2)
  Iyarit Thaipisuttikul (1)
  
  1. Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkok-Noi, Bangkok, 10700, Thailand
  2. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

The Mycobacterium tuberculosis Beijing family is often associated with multidrug resistance and large outbreaks. Conventional genotyping study of a community outbreak of multidrug-resistant tuberculosis (MDR-TB) that occurred in Kanchanaburi Province, Thailand was carried out. The study revealed that the outbreak was clonal and the strain was identified as a member of Beijing family. Although, the outbreak isolates showed identical spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeats patterns, a discrepancy regarding ethambutol resistance was observed. In-depth characterization of the isolates through whole genome sequencing of the first and the last three isolates from our culture collection showed them to belong to principal genetic group 1, single nucleotide polymorphism (SNP) cluster group 2, sequence type 10. Compared with the M. tuberculosis H37Rv reference genome, 1242 SNPs were commonly found in all isolates. The genomes of these isolates were shown to be clonal and highly stable over a 5-year period and two or three unique SNPs were identified in each of the last three isolates. Genes known to be associated with drug resistance and their promoter regions, where applicable, were analyzed. The presence of low or no fitness cost mutations for drug resistance and an additional L731P SNP in the rpoB gene was observed in all isolates. These findings might account for the successful transmission of this MDR-TB strain. Keywords Mycobacterium tuberculosis Multidrug resistance Outbreak Whole genome sequencing Thailand