Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells

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• 作者：Baisheng Long ; Rodiallah Muhamad ; Guokai Yan ; Jie Yu ; Qiwen Fan…
• 关键词：Glutamine ; Lipid metabolism ; Fatty acid ; Quantitative proteomics ; HepG2 cells
• 刊名：Amino Acids
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：48
• 期：5
• 页码：1297-1307
• 全文大小：1,238 KB
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• 作者单位：Baisheng Long (1) (2)
  Rodiallah Muhamad (3)
  Guokai Yan (1) (2)
  Jie Yu (1) (2)
  Qiwen Fan (1) (2)
  Zhichang Wang (1) (2)
  Xiuzhi Li (1) (2)
  Agung Purnomoadi (3)
  Joelal Achmadi (3)
  Xianghua Yan (1) (2)
  
  1. College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China
  2. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China
  3. Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, 50275, Central Java, Indonesia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199
• 卷排序：48

文摘

Glutamine, a multifunctional amino acid, functions in nutrient metabolism, energy balance, apoptosis, and cell proliferation. Lipid is an important nutrient and controls a broad range of physiological processes. Previous studies have demonstrated that glutamine can affect lipolysis and lipogenesis, but the effect of glutamine on the detailed lipid metabolism remains incompletely understood. Here, we applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. The results showed that there were 212 differentially abundant proteins in response to glutamine deprivation, including 150 significantly increased proteins and 62 significantly decreased proteins. Interestingly, functional classification showed that 43 differentially abundant proteins were related to lipid metabolism. Further bioinformatics analysis and western blotting validation revealed that lipid accumulation may be affected by β-oxidation of fatty acid induced by glutamine deprivation in HepG2 cells. Together, our results may provide the potential for regulating lipid metabolism by glutamine in animal production and human nutrition. The MS data have been deposited to the ProteomeXchange Consortium with identifier PXD003387. Keywords Glutamine Lipid metabolism Fatty acid Quantitative proteomics HepG2 cells