Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1

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• 作者：Amélie Segarra ; Florian Mauduit ; Nicole Faury ; Suzanne Trancart…
• 关键词：Interactions ; Ostreid herpesvirus 1 ; Gene expression ; Susceptibility ; Crassostrea gigas
• 刊名：BMC Genomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：1,242 KB
• 参考文献：1. Davison, AJ, Trus, BL, Cheng, N, Steven, AC, Watson, MS, Cunningham, C, Le Deuff, R-M, Renault, T (2005) A novel class of herpesvirus with bivalve hosts. J Gen Virol 86: pp. 41-53 CrossRef
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  9. Schmitt, P, Santini, A, Vergnes, A, Degremont, L, de Lorgeril, J (2013) Sequence polymorphism and expression variability of Crassostrea gigas immune related genes discriminate two oyster lines contrasted in term of resistance to summer mortalities. PLoS One 8: pp. e75900 CrossRef
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  12. Jouaux, A, Lafont, M, Blin, J-L, Houssin, M, Mathieu, M, Lelong, C (2013) Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields. BMC Genomics 14: pp. 590 CrossRef
  13. Fleury, E, Huvet, A (2012) Microarray analysis highlights immune response of Pacific oysters as a determinant of resistance to summer mortality. Mar Biotechnol 14: pp. 203-217 CrossRef
  14. Normand, J, Li, R, Quillien, V, Nicolas, J-L, Boudry, P, Pernet, F, Huvet, A (2014) Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas. Mar Genomics 12: pp. 95-102 CrossRef
  
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant genetic component. Two families with contrasted survival rates were selected. A total of 39 viral genes and five host genes were monitored by real-time PCR. Initial results provided information on (i) the virus cycle of OsHV-1 based on the kinetics of viral DNA replication and transcription and (ii) host defense mechanisms against the virus. Conclusions In the two selected families, the detected amounts of viral DNA and RNA were significantly different. This result suggests that Pacific oysters are genetically diverse in terms of their susceptibility to OsHV-1 infection. This contrasted susceptibility was associated with dissimilar host gene expression profiles. Moreover, the present study showed a positive correlation between viral DNA amounts and the level of expression of selected oyster genes.