Fluorescent protein vectors for pancreatic islet cell identification in live-cell imaging
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- 作者：Hongyan Shuai ; Yunjian Xu ; Qian Yu…
- 关键词：Islets ; α ; cell ; β ; cell ; δ ; cell ; PP ; cell ; Insulin ; Glucagon ; Somatostatin ; Pancreatic polypeptide ; Ca2+ ; cAMP
- 刊名：Pfl¨¹gers Archiv - European Journal of Physiology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：468
- 期：10
- 页码：1765-1777
- 全文大小：2,497 KB
- 刊物主题：Human Physiology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1432-2013
- 卷排序：468
文摘
The islets of Langerhans contain different types of endocrine cells, which are crucial for glucose homeostasis. β- and α-cells that release insulin and glucagon, respectively, are most abundant, whereas somatostatin-producing δ-cells and particularly pancreatic polypeptide-releasing PP-cells are more scarce. Studies of islet cell function are hampered by difficulties to identify the different cell types, especially in live-cell imaging experiments when immunostaining is unsuitable. The aim of the present study was to create a set of vectors for fluorescent protein expression with cell-type-specific promoters and evaluate their applicability in functional islet imaging. We constructed six adenoviral vectors for expression of red and green fluorescent proteins controlled by the insulin, preproglucagon, somatostatin, or pancreatic polypeptide promoters. After transduction of mouse and human islets or dispersed islet cells, a majority of the fluorescent cells also immunostained for the appropriate hormone. Recordings of the sub-plasma membrane Ca2+ and cAMP concentrations with a fluorescent indicator and a protein biosensor, respectively, showed that labeled cells respond to glucose and other modulators of secretion and revealed a striking variability in Ca2+ signaling among α-cells. The measurements allowed comparison of the phase relationship of Ca2+ oscillations between different types of cells within intact islets. We conclude that the fluorescent protein vectors allow easy identification of specific islet cell types and can be used in live-cell imaging together with organic dyes and genetically encoded biosensors. This approach will facilitate studies of normal islet physiology and help to clarify molecular defects and disturbed cell interactions in diabetic islets.