Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus
详细信息 查看全文
- 作者：Hongli Jing ; Longying Gao ; Min Zhang ; Na Wang…
- 关键词：Cell lines ; Goldfish (Carassius auratus) ; Goldfish kidney cell line (GFKf) ; Goldfish snout cell line (GFSe)
- 刊名：Fish Physiology and Biochemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：42
- 期：1
- 页码：303-311
- 全文大小：1,790 KB
- 参考文献：Babu VS, Nambi KS, Chandra V, Ishaq Ahmed VP, Bhonde R, Sahul Hameed AS (2011) Establishment and characterization of a fin cell line from Indian walking catfish, Clarias batrachus (L.). J Fish Dis 34(5):355–364CrossRef PubMed
Becker JA, Tweedie A, Rimmer A, Landos M, Lintermans M, Whittington RJ (2014) Incursions of cyprinid herpesvirus 2 in goldfish populations in Australia despite quarantine practices. Aquaculture 432:53–59CrossRef
Berry ES, Shea TB, Galiks J (1983) Two iridovirus isolates from Carassius auratus (L). J Fish Dis 6:501–510CrossRef
Bols NC, Dayeh VR, Lee LEJ, Schirmer K (2005) Use of fish cell lines in the toxicology and ecotoxicology of fish. In: Moon TW, Mommsen TP (eds) Biochemistry and molecular biology of fishes—environmental toxicology, vol 6. Elsevier, Amsterdam, pp 43–84
Bryson SP, Joyce EM, Martell DJ, LE Lee, Holt SE, Kales SC, Fujiki K, Dixon B, Bols NC (2006) A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes. Mar Biotechnol 8(6):641–653CrossRef PubMed
Earley EM (1975) Chromosome preparations from monolayer cell cultures. Methods Cell Sci 1:31–35
Jeffery KR, Bateman K, Bayley A, Feist SW, Hulland J, Longshaw C, Stone D, Woolford G, Way K (2007) Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK. J Fish Dis 30(11):649–656CrossRef PubMed
Jensen I, Steiro K, Sommer AI, Mennen S, Johansen A, Sandaker EK, Seppola M (2013) Establishing a cell line from Atlantic cod as a novel tool for in vitro studies. Fish Shellfish Immunol 34(1):199–208CrossRef PubMed
Jiang YL, Li ZQ (1987) Isolation of IPN virus from imported rainbow trout (Salmon gairdneri) in P. R. China. J Appl Ichthyol 3(4):191–192CrossRef
Jung SJ, Miyazaki T (1995) Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.). J Fish Dis 18:211–220CrossRef
Lee LEJ, Caldwell SJ, Gibbons J (1997) Development of a cell line from skin of goldfish, Carassius auratus, and effects of ascorbic acid. Histochemical J 29(1):31–43CrossRef
Ohno S, Atkin NB (1966) Comparative DNA values and chromosome complements of eight species of fishes. Chromodoma 18(3):455–466
Ossum CG, Hoffmann EK, Vijayan MM, Holt SE, Bols NC (2004) Characterization of a novel fibroblast like cell line from rainbow trout and responses to sublethal anoxia. J Fish Biol 64(4):1103–1116CrossRef
Ou-Yang ZL, Huang XH, Huang EY, Huang YH, Gong J, Sun JJ, Qin QW (2010) Establishment and characterization of a new marine fish cell line derived from red-spotted grouper Epinephelus akaara. J Fish Biol 77(5):1083–1095CrossRef PubMed
Reed LJ, Muench H (1938) A simple method of estimating fifty percent end points. Am J Epidemiol 27(3):493–497
Rouge´e L, Ostrander GK, Richmond RH, Lu YA (2007) Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder. Dis Aquat Organ 77(2):127–135CrossRef
Sato GH, Sato JD, Okamoto T, McKeehan WL, Barnes DW (2010) Tissue culture: the unlimited potential. Vitro Cell Dev Biol Anim 46(7):590–594CrossRef
Shea TB (1983) Enhancement of goldfish virus type 2 replication by 1-naphthol, the major hydrolysis product of the pesticide carbaryl. Appl Environ Microbiol 46(5):1230–1231PubMedCentral PubMed
Shea TB, Berry ES (1984) Suppression of interferon synthesis by the pesticide carbaryl as a mechanism for enhancement of goldfish virus type-2 replication. Appl Environ Microbiol 47(2):250–252PubMedCentral PubMed
Silas EG, Gopalakrishnan A, Ramachandran A, Anna Mercy TV, Sarkar K, Pushpangadan KR, Anil Kumar P, Ram Mohan MK, Anikuttan KK (2011) Guidelines for green certification of freshwater ornamental fish. The Marine Products Export Development Authority, Kochi
Stephens FJ, Raidal SR, Jones B (2004) Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus. Aust Vet J 82(3):167–169CrossRef PubMed
Stone DM, Ahne W, Denham KL, Dixon PF, Liu CT, Sheppard AM, Taylor GR, Way K (2003) Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups. Dis Aquat Organ 53(3):203–210CrossRef PubMed
Swaminathan TR, Basheer VS, Gopalakrishnan A, Rathore G, Chaudhary DK, Kumar R, Jena JK (2013) Establishment of caudal fin cell lines from tropical ornamental fishes Puntius fasciatus and Pristolepis fasciata endemic to the Western Ghats of India. Acta Trop 128(3):536–541CrossRef PubMed
Tu X, Ling F, Huang A, Wang G (2015) An infection of Gyrodactylus kobayashii Hukuda, 1940 (Monogenea) associated with the mortality of goldfish (Carassius auratus) from central China. Parasitol Res 114(2):737–745CrossRef PubMed
Wang CY (1985) The origin of the goldfish. Bull Biol 12:11–12
Wang XL, Wang N, Sha ZX, Chen SL (2010) Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Fish Physiol Biochem 36(4):1181–1189CrossRef PubMed
Wang N, Wang XL, Sha ZX, Tian YS, Chen SL (2011) Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan and Gilbert. J Fish Dis 34(1):81–85CrossRef PubMed
Wolf K, Quimby MC (1962) Established eurythermic line of fish cells in vitro. Science 135(3508):1065–1066CrossRef PubMed
Xu J, Zeng L, Zhang H, Zhou Y, Ma J, Fan Y (2013) Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China. Vet Microbiol 166(1–2):138–144CrossRef PubMed
Yan W, Nie P, Lu Y (2011) Establishment, characterization and viral susceptibility of a new cell line derived from goldfish, Carassius auratus (L.), tail fin. J Fish Dis 34(10):757–768CrossRef PubMed
Zhang LL, Luo Q, Fang Q, Wang YP (2010) An improved RT–PCR assay for rapid and sensitive detection of grass carp reovirus. J Virol Methods 169(1):28–33CrossRef PubMed
Zhu DM, Yang K, Wang WM (2013) Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala. Fish Physiol Biochem 39(6):1399–1410CrossRef PubMed - 作者单位：Hongli Jing (1)
Longying Gao (2)
Min Zhang (1)
Na Wang (1)
Xiangmei Lin (1)
Lifeng Zhang (3)
Shaoqiang Wu (1)
1. Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China
2. Shekou in Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China
3. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Hydrobiology
Animal Physiology
Animal Anatomy, Morphology and Histology
Animal Biochemistry
Zoology - 出版者：Springer Netherlands
- ISSN：1573-5168
文摘
Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15–20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 106.8 and 105.9 TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future. Keywords Cell lines Goldfish (Carassius auratus) Goldfish kidney cell line (GFKf) Goldfish snout cell line (GFSe)