Molecular phylogeny of pectinase genes PGU in the yeast genus Saccharomyces

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• 作者：G. I. Naumov ; M. Yu. Shalamitskiy ; N. N. Martynenko ; E. S. Naumova
• 关键词：endo ; polygalacturonase ; pectinase ; pectin ; wine yeasts ; Saccharomyces ; phylogenetic analysis ; PGU gene ; introgression
• 刊名：Microbiology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：85
• 期：6
• 页码：717-726
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Pleiades Publishing
• ISSN：1608-3237
• 卷排序：85

文摘

Using yeast genome databases and literature data, phylogenetic analysis of pectinase PGU genes from 112 Saccharomyces strains assigned to the biological species S. arboricola, S. bayanus (var. uvarum), S. cariocanus, S. cerevisiae, S. kudriavzevii, S. mikatae, S. paradoxus, and the hybrid taxon S. pastorianus (syn. S. carlsbergensis) was carried out. A superfamily of divergent PGU genes was found. Natural interspecies transfer of the PGU gene both from S. cerevisiae to S. bayanus and from S. paradoxus to S. cerevisiae may, however, occur. Within the Saccharomyces species, identity of the PGU nucleotide sequences was 98.8–100% for S. cerevisiae, 86.1–95.7% for S. bayanus (var. uvarum), 94–98.3% for S. kudriavzevii, and 96.8–100% for S. paradoxus/S. cariocanus. For the first time, a family of polymeric PGU1b, PGU2b, PGU3b and PGU4b genes is documented for the yeast S. bayanus var. uvarum, a variety important for winemaking.