Recognition of MCF-7 human breast carcinoma cells using silica-encapsulated fluorescent nanoparticles modified with aminophenylboronic acid

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• 作者：Linjing Wu ; Yaqian Yan ; Peiyi Gao ; Shasheng Huang
• 关键词：Sialic acid ; Imaging ; Breast cancer ; Bioconjugation ; MTT assay
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：183
• 期：3
• 页码：1115-1122
• 全文大小：2,373 KB
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• 作者单位：Linjing Wu (1)
  Yaqian Yan (1)
  Peiyi Gao (1)
  Shasheng Huang (1)
  
  1. Life and Environmental Science College, Shanghai Normal University, Shanghai, 200234, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

A method is reported for recognizing MCF-7 human breast carcinoma cells based on silica-encapsulated nanoparticles modified with aminophenylboronic acid which can recognize sialic acid on cell surfaces. Gold@rhodamine B nanoparticles were coated with aminophenylboronic acid and used to capture MCF-7 cells. It is found that the presence of gold NPs was favorable to prepare nanoparticles easily and that they were extraordinarily biocompatible with MCF-7 cells. The experimental results confirmed that the nanoparticles can be used to target breast carcinoma cells using HS 578Bst normal breast cells as the negative control. The MCF-7 cells were imaged by laser scanning microscopy and showed strong red fluorescence in dark field. An MTT test revealed an 82 % viability of cells when 50 mg · mL−1 fluorescent probe was used in the incubation experiments. The results exhibited that the NPs are innocuous and stable. In our perception, the method has a larege potential for early diagnosis of breast cancer due to high affinity between nanoparticles and the breast carcinoma cells.