Evaluation of Inhibition Efficiency for the Detection of Captan, 2,3,7,8-Tetrachlorodibenzodioxin, Pentachlorophenol and Carbosulfan in Water: An Electrochemical Approach

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• 作者：Noel Nesakumar ; Bhat Lakshmishri Ramachandra…
• 关键词：Sensitivity ; Inhibition efficiency ; Acetylcholinesterase ; Michaelis–Menten constant ; Maximum inhibition ; Biosensor
• 刊名：Bulletin of Environmental Contamination and Toxicology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：96
• 期：2
• 页码：217-223
• 全文大小：1,175 KB
• 参考文献：Dhull V, Gahlaunt A, Dilbaghi N, Hooda V (2013) Acetylcholinesterase biosensors for electrochemical detection of organophosphorus compounds: a review. Biochem Res J 2013:1–18
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• 作者单位：Noel Nesakumar (1) (2) (4)
  Bhat Lakshmishri Ramachandra (1) (2) (4)
  Swaminathan Sethuraman (2) (3)
  Uma Maheswari Krishnan (2) (3)
  John Bosco Balaguru Rayappan (1) (2) (4)
  
  1. Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  2. Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  4. School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  3. School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  
• 刊物主题：Pollution, general; Environmental Health; Ecotoxicology; Soil Science & Conservation; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution;
• 出版者：Springer US
• ISSN：1432-0800

文摘

A novel bio-analytical method has been devised based on the change in catalytic activity of acetylcholinesterase (AChE) enzyme induced by captan, carbosulfan, 2,3,7,8-tetrachlorodibenzodioxin (TCDD) and pentachlorophenol (PCP) for the investigation of inhibition efficiency and sensitivity using Pt/ZnO/AChE/Chitosan bioelectrode. The inhibition curves of captan, carbosulfan, TCDD and PCP were similar to Michaelis–Menten curve. TCDD held the minimum inhibitor Michaelis–Menten constant (\(K_{M}^{I}\)) value (10.2 nM) in comparison with PCP (10.9 nM), carbosulfan (14.5 nM) and captan (7.9 × 103 nM). The maximum inhibition of AChE enzyme by captan was about 100 %, which was much higher than that of TCDD (72.7 %), PCP (68.1 %) and carbosulfan (47.7 %). The calculated theoretical sensitivity was in the order of TCDD > PCP > carbosulfan > captan. Comparing with TCDD (35.3 %), PCP (47.8 %) and carbosulfan (20.9 %), only the inhibition efficiency of captan (55.0 %) was the maximum. The developed bioelectrode exhibited high recovery and low relative standard deviation in local tap water samples. Keywords Sensitivity Inhibition efficiency Acetylcholinesterase Michaelis–Menten constant Maximum inhibition Biosensor