Size and shape characterization of hydrated and desiccated exosomes
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- 作者：Vasiliy S. Chernyshev (1)
Rakesh Rachamadugu (2)
Yen Hsun Tseng (1)
David M. Belnap (3)
Yunlu Jia (1)
Kyle J. Branch (1)
Anthony E. Butterfield (1)
Leonard F. Pease III (1) (4) (5)
Philip S. Bernard (2) (6)
Mikhail Skliar (1)
1. Department of Chemical Engineering ; University of Utah ; 50 Central Campus Drive ; Salt Lake City ; UT ; 84112 ; USA
2. Huntsman Cancer Institute ; University of Utah ; University Medical Center ; 2000 Circle of Hope Drive ; Salt Lake City ; UT ; 84112 ; USA
3. Department of Biochemistry and Structural Biology ; University of Utah ; 257S 1400 E ; Salt Lake City ; UT ; 84112 ; USA
4. Division of Gastroenterology ; Hepatology ; and Nutrition ; Department of Internal Medicine ; University of Utah ; Salt Lake City ; UT ; 84112 ; USA
5. Department of Pharmaceutics & Pharmaceutical Chemistry ; University of Utah ; Salt Lake City ; UT ; 84112 ; USA
6. Department of Pathology ; University of Utah ; North Medical Drive East ; Salt Lake City ; UT ; 84112 ; USA - 关键词：Hydrated and desiccated exosomes ; Size and shape characterization
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2015
- 出版时间：May 2015
- 年：2015
- 卷：407
- 期：12
- 页码：3285-3301
- 全文大小：1,743 KB
- 参考文献：1. Thery, C, Zitvogel, L, Amigorena, S (2002) Exosomes: composition, biogenesis and function. Nat Rev Immunol 2: pp. 569-579
2. Trams, EG, Lauter, CJ, Salem, N, Heine, U (1981) Exfoliation of membrane ecto-enzymes in the form of micro-vesicles. Biochim Biophys Acta 645: pp. 63-70 2736(81)90512-5" target="_blank" title="It opens in new window">CrossRef
3. Palma, J (2012) MicroRNAs are exported from malignant cells in customized particles. Nucleic Acids Res 40: pp. 9125-9138 CrossRef
4. King, HW, Michael, MZ, Gleadle, JM (2012) Hypoxic enhancement of exosome release by breast cancer cells. BMC Cancer 12: pp. 421 2407-12-421" target="_blank" title="It opens in new window">CrossRef
5. Kucharzewska, P, Belting, M (2013) Emerging roles of extracellular vesicles in the adaptive response of tumour cells to microenvironmental stress. J Extracell Vesicles.
6. Chevillet, JR (2014) Quantitative and stoichiometric analysis of the microRNA content of exosomes. Proc Natl Acad Sci U S A 111: pp. 14888-14893 CrossRef
7. Gallo, A, Tandon, M, Alevizos, I, Illei, GG (2012) The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PLoS ONE 7: pp. e30679 CrossRef
8. Pucci, F, Pittet, MJ (2013) Molecular pathways: tumor-derived microvesicles and their interactions with immune cells in vivo. Clin Cancer Res 19: pp. 2598-2604
9. Ge, R, Tan, E, Sharghi-Namini, S, Asada, HH (2012) Exosomes in cancer microenvironment and beyond: have we overlooked these extracellular messengers?. Cancer Microenviron 5: pp. 323-332 2307-012-0110-2" target="_blank" title="It opens in new window">CrossRef
10. Zhang, Y (2010) Secreted monocytic miR-150 enhances targeted endothelial cell migration. Mol Cell 39: pp. 133-144 2010.06.010" target="_blank" title="It opens in new window">CrossRef
11. Regev-Rudzki, N (2013) Cell-cell communication between malaria-infected red blood cells via exosome-like vesicles. Cell 153: pp. 1120-1133 2013.04.029" target="_blank" title="It opens in new window">CrossRef
12. Th茅ry, C, Amigorena, S, Raposo, G, Clayton, A (2006) Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol 30: pp. 3.22.1-3.22.29
13. Taylor, DD, Zacharias, W, Gercel-Taylor, C (2011) Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol 728: pp. 235-246 CrossRef
14. Witwer, KW (2013) Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles.
15. Tauro, BJ (2012) Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 56: pp. 293-304 2012.01.002" target="_blank" title="It opens in new window">CrossRef
16. Chen, C (2010) Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip 10: pp. 505-511 CrossRef
17. Vlassov, AV, Magdaleno, S, Setterquist, R, Conrad, R (2012) Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta 1820: pp. 940-948 2012.03.017" target="_blank" title="It opens in new window">CrossRef
18. Rekker, K (2014) Comparison of serum exosome isolation methods for microRNA profiling. Clin Biochem 47: pp. 135-138 2013.10.020" target="_blank" title="It opens in new window">CrossRef
19. Alvarez, ML, Khosroheidari, M, Kanchi Ravi, R, DiStefano, JK (2012) Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int 82: pp. 1024-1032 2012.256" target="_blank" title="It opens in new window">CrossRef
20. Gy枚rgy, B (2011) Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. Cell Mol Life Sci 68: pp. 2667-2688 CrossRef
21. Pol, E, Coumans, F, Varga, Z, Krumrey, M, Nieuwland, R (2013) Innovation in detection of microparticles and exosomes. J Thromb Haemost 11: pp. 36-45
22. Pol, E (2010) Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost 8: pp. 2596-2607 2010.04074.x" target="_blank" title="It opens in new window">CrossRef
23. Thery, C, Ostrowski, M, Segura, E (2009) Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 9: pp. 581-593 2567" target="_blank" title="It opens in new window">CrossRef
24. Bacher, G (2001) Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. J Mass Spectrom 36: pp. 1038-1052 2/jms.208" target="_blank" title="It opens in new window">CrossRef
25. Caulfield, MP (2008) Direct determination of lipoprotein particle sizes and concentrations by ion mobility analysis. Clin Chem 54: pp. 1307-1316 2007.100586" target="_blank" title="It opens in new window">CrossRef
26. Guha, S, Pease, LF, Brorson, KA, Tarlov, MJ, Zachariah, MR (2011) Evaluation of electrospray differential mobility analysis for virus particle analysis: potential applications for biomanufacturing. J Virol Methods 178: pp. 201-208 2011.09.012" target="_blank" title="It opens in new window">CrossRef
27. Guha, S, Li, M, Tarlov, MJ, Zachariah, MR (2012) Electrospray-differential mobility analysis of bionanoparticles. Trends Biotechnol 30: pp. 291-300 2012.02.003" target="_blank" title="It opens in new window">CrossRef
28. Pease, LF (2010) Packing and size determination of colloidal nanoclusters. Langmuir 26: pp. 11384-11390 21/la100839t" target="_blank" title="It opens in new window">CrossRef
29. Wiedensohler, A (1988) An approximation of the bipolar charge distribution for particles in the submicron size range. J Aerosol Sci 19: pp. 387-389 21-8502(88)90278-9" target="_blank" title="It opens in new window">CrossRef
30. Flagan, RC (2008) Differential mobility analysis of aerosols: a tutorial. KONA Powder Part J 26: pp. 254-268 2008023" target="_blank" title="It opens in new window">CrossRef
31. Lattin, JR, Belnap, DM, Pitt, WG (2012) Formation of eLiposomes as a drug delivery vehicle. Colloids Surf B 89: pp. 93-100 2011.08.030" target="_blank" title="It opens in new window">CrossRef
32. Belnap, DM, Grochulski, WD, Olson, NH, Baker, TS (1993) Use of radial density plots to calibrate image magnification for frozen-hydrated specimens. Ultramicroscopy 48: pp. 347-358 CrossRef
33. Deegan, RD (1997) Capillary flow as the cause of ring stains from dried liquid drops. Nature 389: pp. 827-829 27" target="_blank" title="It opens in new window">CrossRef
34. Nguyen, TAH, Hampton, MA, Nguyen, AV (2013) Evaporation of nanoparticle droplets on smooth hydrophobic surfaces: the inner coffee ring deposits. J Phys Chem C 117: pp. 4707-4716 21/jp3126939" target="_blank" title="It opens in new window">CrossRef
35. Yunker, PJ, Still, T, Lohr, MA, Yodh, AG (2011) Suppression of the coffee-ring effect by shape-dependent capillary interactions. Nature 476: pp. 308-311 CrossRef
36. Still, T, Yunker, PJ, Yodh, AG (2012) Surfactant-induced Marangoni eddies alter the coffee-rings of evaporating colloidal drops. Langmuir 28: pp. 4984-4988 21/la204928m" target="_blank" title="It opens in new window">CrossRef
37. Kaufman, SL, Skogen, JW, Dorman, FD, Zarrin, F, Lewis, KC (1996) Macromolecule analysis based on electrophoretic mobility in air: globular proteins. Anal Chem 68: pp. 1895-1904 21/ac951128f" target="_blank" title="It opens in new window">CrossRef
38. Gy枚rgy, B (2011) Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 117: pp. e39-e48 2/blood-2010-09-307595" target="_blank" title="It opens in new window">CrossRef
39. Deun, J (2014) The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles.
40. Caradec, J (2014) Reproducibility and efficiency of serum-derived exosome extraction methods. Clin Biochem 47: pp. 1286-1292 2014.06.011" target="_blank" title="It opens in new window">CrossRef
41. Jeppesen, DK (2014) Comparative analysis of discrete exosome fractions obtained by differential centrifugation. J Extracell Vesicles.
42. Mathivanan, S, Simpson, RJ (2009) ExoCarta: a compendium of exosomal proteins and RNA. Proteomics 9: pp. 4997-5000 2/pmic.200900351" target="_blank" title="It opens in new window">CrossRef
43. Laulagnier, K (2005) Characterization of exosome subpopulations from RBL-2H3 cells using fluorescent lipids. Blood Cells Mol Dis 35: pp. 116-121 2005.05.010" target="_blank" title="It opens in new window">CrossRef
44. Bobrie, A, Colombo, M, Krumeich, S, Raposo, G, Th茅ry, C (2012) Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles.
45. Yellon, DM, Davidson, SM (2014) Exosomes: nanoparticles involved in cardioprotection?. Circ Res 114: pp. 325-332 CrossRef
46. Kobayashi, M (2014) Ovarian cancer cell invasiveness is associated with discordant exosomal sequestration of Let-7 miRNA and miR-200. J Transl Med 12: pp. 4 2-4" target="_blank" title="It opens in new window">CrossRef
47. Petersen, KE (2014) A review of exosome separation techniques and characterization of B16-F10 mouse melanoma exosomes with AF4-UV-MALS-DLS-TEM. Anal Bioanal Chem 406: pp. 7855-7866 216-014-8040-0" target="_blank" title="It opens in new window">CrossRef
48. Sharma, S, Gillespie, BM, Palanisamy, V, Gimzewski, JK (2011) Quantitative nanostructural and single-molecule force spectroscopy biomolecular analysis of human-saliva-derived exosomes. Langmuir 27: pp. 14394-14400 21/la2038763" target="_blank" title="It opens in new window">CrossRef
49. Sharma, S (2010) Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy. ACS Nano 4: pp. 1921-1926 21/nn901824n" target="_blank" title="It opens in new window">CrossRef
50. Sharma, S, Das, K, Woo, J, Gimzewski, JK (2014) Nanofilaments on glioblastoma exosomes revealed by peak force microscopy. J R Soc Interface 11: pp. 20131150 2013.1150" target="_blank" title="It opens in new window">CrossRef
51. Conde-Vancells, J (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res 7: pp. 5157-5166 21/pr8004887" target="_blank" title="It opens in new window">CrossRef
52. Zhou, Y (2013) Exosomes released by human umbilical cord mesenchymal stem cells protect against cisplatin-induced renal oxidative stress and apoptosis in vivo and in vitro. Stem Cell Res Ther 4: pp. 34 CrossRef
53. Coleman, BM, Hanssen, E, Lawson, VA, Hill, AF (2012) Prion-infected cells regulate the release of exosomes with distinct ultrastructural features. FASEB J 26: pp. 4160-4173 202077" target="_blank" title="It opens in new window">CrossRef
54. Pol, E, B枚ing, AN, Harrison, P, Sturk, A, Nieuwland, R (2012) Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev 64: pp. 676-705 24/pr.112.005983" target="_blank" title="It opens in new window">CrossRef
55. Momen-Heravi, F (2012) Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles. Front Physiol 3: pp. 162
56. Sokolova, V (2011) Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids Surf B 87: pp. 146-150 2011.05.013" target="_blank" title="It opens in new window">CrossRef
57. Tscharnuter, WT (2006) Photon correlation spectroscopy in particle sizing. In Encyclopedia of analytical chemistry. Wiley, Hoboken
58. Besseling, NAM (1997) Theory of hydration forces between surfaces. Langmuir 13: pp. 2113-2122 21/la960672w" target="_blank" title="It opens in new window">CrossRef
59. He, L, Hu, Y, Wang, M, Yin, Y (2012) Determination of solvation layer thickness by a magnetophotonic approach. ACS Nano 6: pp. 4196-4202 21/nn3007288" target="_blank" title="It opens in new window">CrossRef
60. Tathireddy, P, Choi, Y-H, Skliar, M (2008) Particle AC electrokinetics in planar interdigitated microelectrode geometry. J Electrostat 66: pp. 609-619 2008.09.002" target="_blank" title="It opens in new window">CrossRef
61. Iyer, S, Gaikwad, RM, Subba-Rao, V, Woodworth, CD, Sokolov, I (2009) Atomic force microscopy detects differences in the surface brush of normal and cancerous cells. Nat Nanotechnol 4: pp. 389-393 2009.77" target="_blank" title="It opens in new window">CrossRef
62. Frank, J (2002) Single-particle imaging of macromolecules by cryo-electron microscopy. Annu Rev Biophys Biomol Struct 31: pp. 303-319 2901.134202" target="_blank" title="It opens in new window">CrossRef
63. Oh, E (2011) Cellular uptake and fate of PEGylated gold nanoparticles is dependent on both cell-penetration peptides and particle size. ACS Nano 5: pp. 6434-6448 21/nn201624c" target="_blank" title="It opens in new window">CrossRef
64. Lavialle, F (2009) Nanovesicles released by Dictyostelium cells: a potential carrier for drug delivery. Int J Pharm 380: pp. 206-215 2009.06.039" target="_blank" title="It opens in new window">CrossRef
65. Varga, Z (2014) Towards traceable size determination of extracellular vesicles. J Extracell Vesicles.
66. Hardij, J (2013) Characterisation of tissue factor-bearing extracellular vesicles with AFM: comparison of air-tapping-mode AFM and liquid peak force AFM. J Extracell Vesicles.
67. Dragovic, RA (2011) Sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis. Nanomedicine 7: pp. 780-788 2011.04.003" target="_blank" title="It opens in new window">CrossRef
68. Momen-Heravi, F (2012) Alternative methods for characterization of extracellular vesicles. Front Physiol 3: pp. 354
69. Hood, JL, Pan, H, Lanza, GM, Wickline, SA (2009) Paracrine induction of endothelium by tumor exosomes. Lab Investig 89: pp. 1317-1328 2009.94" target="_blank" title="It opens in new window">CrossRef
70. Atay, S, Gercel-Taylor, C, Kesimer, M, Taylor, DD (2011) Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells. Exp Cell Res 317: pp. 1192-1202 2011.01.014" target="_blank" title="It opens in new window">CrossRef
71. Tian, T, Wang, Y, Wang, H, Zhu, Z, Xiao, Z (2010) Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy. J Cell Biochem 111: pp. 488-496 2/jcb.22733" target="_blank" title="It opens in new window">CrossRef
72. Tatischeff, I, Larquet, E, Falc贸n-P茅rez, JM, Turpin, P-Y, Kruglik, SG (2012) Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy. J Extracell Vesicles.
73. Wahlgren, J, Karlson, TDL, Glader, P, Telemo, , Valadi, H (2012) Activated human T cells secrete exosomes that participate in IL-2 mediated immune response signaling. PLoS ONE 7: pp. e49723 23" target="_blank" title="It opens in new window">CrossRef
74. Ng, YH (2013) Endometrial exosomes/microvesicles in the uterine microenvironment: a new paradigm for embryo-endometrial cross talk at implantation. PLoS ONE 8: pp. e58502 2" target="_blank" title="It opens in new window">CrossRef - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Food Science
Inorganic Chemistry
Physical Chemistry
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Berlin / Heidelberg
- ISSN：1618-2650
文摘
Exosomes are stable nanovesicles secreted by cells into the circulation. Their reported sizes differ substantially, which likely reflects the difference in the isolation techniques used, the cells that secreted them, and the methods used in their characterization. We analyzed the influence of the last factor on the measured sizes and shapes of hydrated and desiccated exosomes isolated from the serum of a pancreatic cancer patient and a healthy control. We found that hydrated exosomes are close-to-spherical nanoparticles with a hydrodynamic radius that is substantially larger than the geometric size. For desiccated exosomes, we found that the desiccated shape and sizing are influenced by the manner in which drying occurred. Isotropic desiccation in aerosol preserves the near-spherical shape of the exosomes, whereas drying on a surface likely distorts their shapes and influences the sizing results obtained by techniques that require surface fixation prior to analysis.