Differences in metabolomic and transcriptomic profiles between responders and non-responders to an n-3 polyunsaturated fatty acids (PUFAs) supplementation
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- 作者：Iwona Rudkowska (1) (2)
Ann-Marie Paradis (1)
Elisabeth Thifault (1)
Pierre Julien (2)
Olivier Barbier (2)
Patrick Couture (1)
Simone Lemieux (1)
Marie-Claude Vohl (1) (2) - 关键词：Lipidomics ; Metabolic pathways ; Metabolites ; Microarray ; Nutrigenomics
- 刊名：Genes & Nutrition
- 出版年：2013
- 出版时间：July 2013
- 年：2013
- 卷：8
- 期：4
- 页码：411-423
- 全文大小：227KB
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Ann-Marie Paradis (1)
Elisabeth Thifault (1)
Pierre Julien (2)
Olivier Barbier (2)
Patrick Couture (1)
Simone Lemieux (1)
Marie-Claude Vohl (1) (2)
1. Institute of Nutraceuticals and Functional Foods (INAF), Laval University, Pavillon des Services, 2440, Boulevard Hochelaga, Quebec, QC, G1V 0A6, Canada
2. Endocrinology and Genomics, Laval University Medical Center, Quebec, QC, Canada - ISSN：1865-3499
文摘
Studies have demonstrated large within-population heterogeneity in plasma triacylglycerol (TG) response to n-3 PUFA supplementation. The objective of the study was to compare metabolomic and transcriptomic profiles of responders and non-responders of an n-3 PUFA supplementation. Thirty subjects completed a 2-week run-in period followed by a 6-week supplementation with n-3 PUFA (3?g/d). Six subjects did not lower their plasma TG (+9?%) levels (non-responders) and were matched to 6 subjects who lowered TG (?1?%) concentrations (responders) after the n-3 PUFA supplementation. Pre-n-3 PUFA supplementation characteristics did not differ between the non-responders and responders except for plasma glucose concentrations. In responders, changes were observed for plasma hexose concentrations, docosahexaenoic acid, stearoyl-CoA-desaturase-18 ratio, and the extent of saturation of glycerophosphatidylcholine after n-3 PUFA supplementation; however, no change in these parameters was observed in non-responders. Transcriptomic profiles after n-3 PUFA supplementation indicate changes in glycerophospholipid metabolism in both subgroups and sphingolipid metabolism in non-responders. Six key genes in lipid metabolism: fatty acid desaturase 2, phospholipase A2 group IVA, arachidonate 15-lipoxygenase, phosphatidylethanolamine N-methyltransferase, monoglyceride lipase, and glycerol-3-phosphate acyltransferase, were expressed in opposing direction between subgroups. In sum, results highlight key differences in lipid metabolism of non-responders compared to responders after an n-3 PUFA supplementation, which may explain the inter-individual variability in plasma TG response.