Control of enzymatic degradation of biodegradable polymers by treatment with biosurfactants, mannosylerythritol lipids, derived from Pseudozyma spp. yeast strains

详细信息    查看全文

• 作者：Tokuma Fukuoka ; Yukiko Shinozaki ; Wataru Tsuchiya…
• 关键词：Biosurfactants ; Biodegradable plastics ; Pseudozyma yeast ; Mannosylerythritol lipid ; PaE
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：100
• 期：4
• 页码：1733-1741
• 全文大小：755 KB
• 参考文献：Espino-Rammer L, Ribitsch D, Przylucka A, Marold A, Greimel KJ, Herrero Acero E, Guebitz GM, Kubicek CP, Druzhinina IS (2013) Two novel class II hydrophobins from Trichoderma spp. stimulate enzymatic hydrolysis of poly(ethylene terephthalate) when expressed as fusion proteins. Appl Environ Microbiol 79:4230–4238. doi:10.​1128/​AEM.​01132-13 PubMedCentral CrossRef PubMed
  Fukuoka T, Yoshida S, Nakamura J, Koitabashi M, Sakai H, Abe M, Kitamoto D, Kitamoto H (2015) Application of yeast glycolipid biosurfactant, mannosylerythritol lipid, as agrospreaders. J Oleo Sci 64:689–695. doi:10.​5650/​jos.​ess15017 CrossRef PubMed
  Kitamoto D, Akiba S, Hioki C, Tabuchi T (1990a) Extracellular accumulation of mannosylerythritol lipids by a strain of Candida antarctica. Agric Biol Chem 54:31–36. doi:10.​1080/​00021369.​1990.​10869918 CrossRef
  Kitamoto D, Haneishi K, Nakahara T, Tabuchi T (1990b) Production of mannosylerythritol lipids by Candida antarctica from vegetable oils. Agric Biol Chem 54:37–40. doi:10.​1080/​00021369.​1990.​10869919 CrossRef
  Kitamoto D, Morita T, Fukuoka T, Konishi M, Imura T (2009) Self-assembling properties of glycolipid biosurfactants and their potential applications. Curr Opin Colloid Interface Sci 14:315–328. doi:10.​1016/​j.​concis.​2009.​05.​009 CrossRef
  Kitamoto HK, Shinozaki Y, Cao XH, Morita T, Konishi M, Tago K, Kajiwara H, Koitabashi M, Yoshida S, Watanabe T, Sameshima-Yamashita Y, Nakajima-Kambe T, Tsushima S (2011) Phyllosphere yeasts rapidly break down biodegradable plastics. AMB Express 1:44. doi:10.​1186/​2191-0855-1-44 PubMedCentral CrossRef PubMed
  Kyrikou I, Briassoulis D (2007) Biodegradation of agricultural plastic films: a critical review. J Polym Environ 15:125–150. doi:10.​1007/​s10924-007-0053-8 CrossRef
  Morita T, Konishi M, Fukuoka T, Imura T, Kitamoto D (2007) Physiological differences in the formation of the glycolipid biosurfactants, mannosylerythritol lipids, between Pseudozyma antarctica and Pseudozyma aphidis. Appl Microbiol Biotechnol 74:307–315. doi:10.​1007/​s00253-006-0672-3 CrossRef PubMed
  Morita T, Fukuoka T, Imura T, Kitamoto D (2013) Production of mannosylerythritol lipids and their application in cosmetics. Appl Microbiol Biotechnol 97:4691–4700. doi:10.​1007/​s00253-013-4858-1 CrossRef PubMed
  Shinozaki Y, Morita T, Cao XH, Yoshida S, Koitabashi M, Watanabe T, Suzuki K, Sameshima-Yamashita Y, Nakajima-Kambe T, Fujii T, Kitamoto HK (2013a) Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. Appl Microbiol Biotechnol 97:2951–2959. doi:10.​1007/​s00253-012-4188-8 CrossRef PubMed
  Shinozaki Y, Watanabe T, Suzuki K, Nakajima-Kambe T, Kitamoto HK (2013b) Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films. J Biosci Bioeng 115:111–114. doi:10.​1016/​j.​jbiosc.​2012.​08.​010 CrossRef PubMed
  Shinozaki Y, Kikkawa Y, Sato S, Fukuoka T, Watanabe T, Yoshida S, Nakajima-Kambe T, Kitamoto HK (2013c) Enzyme degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic microscopy study. Appl Microbiol Biotechnol 97:8591–8598. doi:10.​1007/​s00253-012-4673-0 CrossRef PubMed
  Suzuki K, Sakamoto H, Shinozaki Y, Tabata J, Watanabe T, Mochizuki A, Koitabashi M, Fujii T, Tsushima S, Kitamoto HK (2013) Affinity purification and characterization of a biodegradable plastic-degrading enzyme from a yeast isolated from the larval midgut of a stag beetle, Aegus laevicollis. Appl Microbiol Biotechnol 97:7679–7688. doi:10.​1007/​s00253-012-4595-x CrossRef PubMed
  Takahashi T, Maeda H, Yoneda S, Ohtaki S, Yamagata Y, Hasegawa F, Gomi K, Nakajima T, Abe K (2005) The fungal hydrophobin RolA recruits polyesterase and laterally moves on hydrophobic surface. Mol Microbiol 57:1780–1796. doi:10.​1111/​j.​1365-2958.​2005.​ 04803.​x CrossRef PubMed
  Ueda H, Mitsuhara I, Tabata J, Kugimiya S, Watanabe T, Suzuki K, Yoshida S, Kitamoto H (2015) Extracellular esterases of phylloplane yeast Pseudozyma antarctica induce defect on cuticle layer structure and water-holding ability of plant leaves. Appl Microbiol Biotechnol 99:6405–6415. doi:10.​1007/​s00253-015-6523-3 CrossRef PubMed
  Ueno Y, Inoh Y, Furuno T, Hirashima N, Kitamoto D, Nakanishi M (2007) NBD-conjugated biosurfactant (MEL-A) shows a new pathway for transfection. J Control Release 123:247–253. doi:10.​1016/​j.​jconrel.​2007.​08.​012 CrossRef PubMed
  Watanabe T, Shinozaki Y, Yoshida S, Koitabashi M, Sameshima-Yamashita Y, Fujii T, Fukuoka T, Kitamoto HK (2014) Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics. J Biosci Bioeng 117:325–329. doi:10.​1016/​j.​jbiosc.​2013.​09.​ 002 CrossRef PubMed
  Yamamoto-Tamura K, Hiradate S, Watanabe T, Koitabashi M, Sameshima-Yamashita Y, Yarimizu T, Kitamoto H (2015) Contribution of soil esterase to biodegradation of aliphatic polyester agricultural mulch film in cultivated soils. AMB Express 5:10. doi:10.​1186/​s13568-014-0088-x PubMedCentral CrossRef PubMed
  
• 作者单位：Tokuma Fukuoka (1)
  Yukiko Shinozaki (2)
  Wataru Tsuchiya (3)
  Ken Suzuki (2)
  Takashi Watanabe (2)
  Toshimasa Yamazaki (3)
  Dai Kitamoto (1)
  Hiroko Kitamoto (2)
  
  1. Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan
  2. National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kan-nondai, Tsukuba, Ibaraki, 305-8604, JAPAN
  3. Biomolecular Research Unit, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kan-nondai, Tsukuba, Ibaraki, 305-8602, JAPAN
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Cutinase-like esterase from the yeasts Pseudozyma antarctica (PaE) shows strong degradation activity in an agricultural biodegradable plastic (BP) model of mulch films composed of poly(butylene succinate-co-adipate) (PBSA). P. antarctica is known to abundantly produce a glycolipid biosurfactant, mannosylerythritol lipid (MEL). Here, the effects of MEL on PaE-catalyzed degradation of BPs were investigated. Based on PBSA dispersion solution, the degradation of PBSA particles by PaE was inhibited in the presence of MEL. MEL behavior on BP substrates was monitored by surface plasmon resonance (SPR) using a sensor chip coated with polymer films. The positive SPR signal shift indicated that MEL readily adsorbed and spread onto the surface of a BP film. The amount of BP degradation by PaE was monitored based on the negative SPR signal shift and was decreased 1.7-fold by MEL pretreatment. Furthermore, the shape of PBSA mulch films in PaE-containing solution was maintained with MEL pretreatment, whereas untreated films were almost completely degraded and dissolved. These results suggest that MEL covering the surface of BP film inhibits adsorption of PaE and PaE-catalyzed degradation of BPs. We applied the above results to control the microbial degradation of BP mulch films. MEL pretreatment significantly inhibited BP mulch film degradation by both PaE solution and BP-degradable microorganism. Moreover, the degradation of these films was recovered after removal of the coated MEL by ethanol treatment. These results demonstrate that the biodegradation of BP films can be readily and reversibly controlled by a physical approach using MEL. Keywords Biosurfactants Biodegradable plastics Pseudozyma yeast Mannosylerythritol lipid PaE