Allelic variation in PtoPsbW associated with photosynthesis, growth, and wood properties in Populus tomentosa
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- 作者：Longxin Wang ; Bowen Wang ; Qingzhang Du ; Jinhui Chen…
- 关键词：Photosystem ; Photosynthesis ; PsbW protein ; Single ; nucleotide polymorphisms ; Haplotype ; based association analyses ; Linkage disequilibrium ; Populus tomentosa
- 刊名：Molecular Genetics and Genomics
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：292
- 期：1
- 页码：77-91
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Cell Biology; Biochemistry, general; Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics;
- 出版者：Springer Berlin Heidelberg
- ISSN：1617-4623
- 卷排序：292
文摘
Photosynthesis is one of the most important reactions on earth. PsbW, a nuclear-encoded subunit of photosystem II (PSII), stabilizes PSII structure and plays an important role in photosynthesis. Here, we used candidate gene-based linkage disequilibrium (LD) mapping to detect significant associations between allelic variations of PtoPsbW and traits related to photosynthesis, growth, and wood properties in Populus tomentosa. PtoPsbW showed the highest expression in leaves and it increased during the development of these leaves, suggesting that PtoPsbW may play an important role in plant growth and development. Analysis of nucleotide diversity and LD revealed that PtoPsbW has low single-nucleotide polymorphism (SNP) diversity (πtot = 0.0048 and θw = 0.0050) and relatively low average value of LD (0.1500), indicating that PtoPsbW is conserved due to its indispensable function. Using single-SNP associations in an association population of 435 individuals, we identified five significant associations at the threshold of P ≤ 0.05, explaining 3.28–15.98 % of the phenotypic variation. Haplotype-based association analyses indicated that 13 haplotypes (P ≤ 0.05) from six blocks were associated with photosynthesis, growth, and wood properties. Our work shows that identifying allelic variation and LD can help to decipher the genetic basis of photosynthesis and could potentially be applied for molecular marker-assisted selection in Populus.