Co-application of 6-ketone type brassinosteroid and metal chelator alleviates cadmium toxicity in B. juncea L.

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• 作者：Ravdeep Kaur ; Poonam Yadav…
• 关键词：Brassinosteroids ; Phytoextraction ; Citric acid ; Cadmium ; Oxidative stress ; B. juncea
• 刊名：Environmental Science and Pollution Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：24
• 期：1
• 页码：685-700
• 全文大小：
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water M
• 出版者：Springer Berlin Heidelberg
• ISSN：1614-7499
• 卷排序：24

文摘

Plant growth regulator-assisted phytoremediation has been assessed as a novel strategy to improve phytoremediation potential of plants. In the present work, potential of castasterone, a plant growth regulator, combined with citric acid was explored for phytoremediation of cadmium in Brassica juncea seedlings. The seedlings were raised under controlled laboratory conditions for 7 days. Results revealed that 0.6 mM cadmium exposure induced toxicity in the seedlings, which was reflected through root growth inhibition, accumulation of hydrogen peroxide and malondialdehyde, and loss of cell viability. Pre-sowing treatment of castasterone supplemented with citric acid enhanced cadmium accumulation in the roots (from 752 μg/g DW to 1192 μg/g DW) and shoots (from 88 μg/g DW to 311 μg/g DW) and also improved root length, shoot length, fresh weight, and dry weight of seedlings by 81, 17, 39, and 35 %, respectively. The co-application reduced malondialdehyde accumulation by 39 % and reduced oxidative stress by enhancing the activities of antioxidant enzymes (superoxide dismutase, guaiacol peroxidase, catalase, ascorbate peroxidase, dehydroascorbate, glutathione reductase, glutathione peroxidase, glutathione-S-transferase, polyphenol oxidase), maximum enhancement (82 %) being in polyphenol oxidase. Similarly, the contents of water- and lipid-soluble antioxidants were found to increase by 31 and 4 %, respectively. Confocal microscopy revealed enhanced content of NO. Results suggested that binary combination of castasterone and citric acid is helpful in improving cadmium accumulation and ameliorating metal toxicity in B. juncea seedlings.