Maternal intake of methyl-group donors affects DNA methylation of metabolic genes in infants

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• 作者：Sara Pauwels ; Manosij Ghosh ; Radu Corneliu Duca ; Bram Bekaert…
• 关键词：Methyl ; group donors ; DNA methylation ; LEP ; IGF2 DMR ; RXRA ; DNMT1 ; Lactation ; Pregnancy
• 刊名：Clinical Epigenetics
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：9
• 期：1
• 全文大小：729KB
• 刊物主题：Human Genetics; Gene Function;
• 出版者：BioMed Central
• ISSN：1868-7083
• 卷排序：9

文摘

BackgroundMaternal nutrition during pregnancy and infant nutrition in the early postnatal period (lactation) are critically involved in the development and health of the newborn infant. The Maternal Nutrition and Offspring’s Epigenome (MANOE) study was set up to assess the effect of maternal methyl-group donor intake (choline, betaine, folate, methionine) on infant DNA methylation. Maternal intake of dietary methyl-group donors was assessed using a food-frequency questionnaire (FFQ). Before and during pregnancy, we evaluated maternal methyl-group donor intake through diet and supplementation (folic acid) in relation to gene-specific (IGF2 DMR, DNMT1, LEP, RXRA) buccal epithelial cell DNA methylation in 6 months old infants (n = 114) via pyrosequencing. In the early postnatal period, we determined the effect of maternal choline intake during lactation (in mothers who breast-fed for at least 3 months) on gene-specific buccal DNA methylation (n = 65).